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SPATS2L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPATS2L Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113154-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATS2L (NM_015535), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNAPTP6 | SPATS2L | SPATS2-like protein | SGNP
Origin Species
Human
NCBI GenBank
NM_015535
SwissProt
Q9NUQ6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
61.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAELNTHVNV KEKIYAVRSV VPNKSNNEIV LVLQQFDFNV DKAVQAFVDG SAIQVLKEWN
61MTGKKKNNKR KRSKSKQHQG NKDAKDKVER PEAGPLQPQP PQIQNGPMNG CEKDSSSTDS
121ANEKPALIPR EKKISILEEP SKALRGVTEG NRLLQQKLSL DGNPKPIHGT TERSDGLQWS
181AEQPCNPSKP KAKTSPVKSN TPAAHLEIKP DELAKKRGPN IEKSVKDLQR CTVSLTRYRV
241MIKEEVDSSV KKIKAAFAEL HNCIIDKEVS LMAEMDKVKE EAMEILTARQ KKAEELKRLT
301DLASQMAEMQ LAELRAEIKH FVSERKYDEE LGKAARFSCD IEQLKAQIML CGEITHPKNN
361YSSRTPCSSL LPLLNAHAAT SGKQSNFSRK SSTHNKPSEG KAANPKMVSS LPSTADPSHQ
421TMPANKQNGS SNQRRRFNPQ YHNNRLNGPA KSQGSGNEAE PLGKGNSRHE HRRQPHNGFR
481PKNKGGAKNQ EASLGMKTPE APAHSEKPRR RQHAADTSEA RPFRGSVGRV SQCNLCPTRI
541EVSTDAAVLS VPAVTLVA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

558 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.