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SPATS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPATS2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110191-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATS2 (NM_023071), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Nbla00526 | p59SCR | SPATA10 | SCR59 | SPATS2
Origin Species
Human
NCBI GenBank
NM_023071
SwissProt
Q86XZ4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MSRKQNQKDS SGFIFDLQSN TVLAQGGAFE NMKEKINAVR AIVPNKSNNE IILVLQHFDN
61CVDKTVQAFM EGSASEVLKE WTVTGKKKNK KKKNKPKPAA EPSNGIPDSS KSVSIQEEQS
121APSSEKGGMN GYHVNGAIND TESVDSLSEG LETLSIDARE LEDPESAMLD TLDRTGSMLQ
181NGVSDFETKS LTMHSIHNSQ QPRNAAKSLS RPTTETQFSN MGMEDVPLAT SKKLSSNIEK
241SVKDLQRCTV SLARYRVVVK EEMDASIKKM KQAFAELESC LMDREVALLA EMDKVKAEAM
301EILLSRQKKA ELLKKMTHVA VQMSEQQLVE LRADIKHFVS ERKYDEDLGR VARFTCDVET
361LKKSIDSFGQ VSHPKNSYST RSRCSSVTSV SLSSPSDASA ASSSTCASPP SLTSANKKNF
421APGETPAAIA NSSGQPYQPL REVLPGNRRG GQGYRPQGQK SNDPMNQGRH DSMGRYRNSS
481WYSSGSRYQS APSQAPGNTI ERGQTHSAGT NGTGVSMEPS PPTPSFKKGL PQRKPRTSQT
541EAVNS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

545 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.