Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPATA6 / SRF-1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111467-100
Catalog Number: LS-G111467
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATA6 (NM_019073), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HASH | SPATA6 | SRF1 | Spermatogenesis associated 6 | SRF-1
- Origin Species
- Human
- NCBI GenBank
- NM_019073
- SwissProt
- Q9NWH7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPKVKALQCA LALEISSVTC PGVVLKDKED IYLSICVFGQ YKKTQCVPAT FPLVFNARMV61FEKVFPDAVD PGDVVTQLEY DTAVFELIQL VPPVGETLST YDENTRDFMF PGPNQMSGHH121DSNRQVTMRR ISGLRGNAPR LEFSTTSVIT ECLISSRKCH TQDKFIYHLA PVEKSHGRLQ181NRTSRSQKKK SKSPERSKYC INAKNYEQPT ISSKSHSPSP YTKRRMCELS EDTRRRLAHL241NLGPYEFKKE TDKPPFVIRH VDPPSPRADT LLGSSGRDCE RDGWSRVHND HSHLGCCRPK301DYKVIRTPHG RDFDDSLEKC EEYLSPRSCS KPRHSARTLL VHSAPSTMPK HSPSPVLNRA361SLRERFHSDW CSPSNCDEIH DRVKNVLKSH QAHQRHLYDE RDLEKDDELE LKRSLLCRDS421AYDSDPEYSS CQQPRGTFHL DDGEYWSNRA ASYKGKSHRP IFENSMDKMY RNLYKKACSS481ASHTQESFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
488 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.