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SPATA31D4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPATA31D4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SPATA31D4 antibodies →
$725.00each
Catalog Number: LS-G126576-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATA31D4 (NM_001145197), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001145197

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
102.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENILCFLNS YTETGLSPDS HCLDIDLNFI CLSGLGLFIL YLFYMVLTLY SSPTEKNNDT
61QKHQGRARRK RKSVTFKDRK SLQKEAEEER KLHSFLKSFG PPVSCSPLGQ HHDTTLFRRL
121LCPDPVCRVC NRATADIQRL LSWESLKDAA PSVSPLASSA SGAESSFTLA STPSATTPED
181LILSSRPKPS PPPPLILSPD LITTLADLFS PSPLRDPLPP QPVSPLDSKF PIDHSPPQQL
241PFPLLPPHHI ERVEPSLQPE ASLSLNTIFS FGSTLCQDIS QAVNRTDSCA RHHGPPTPSA
301LPPEDCTVTQ SKSNLTVLKT FPEMLSLGGS GGSSTSAPTT KGIDHSCPAS SEFSWWQPHA
361KDSFSSNFVP SDFMEELLTL HSSEASLGGH SVANIIQPVN ISFLSHDIPA LLERQVKRRG
421DFLMWKENGK KPGSFPTQLR PNYQLNSSRN MLTSTAVKHD LAESFPFWAS KGKLEWQHIH
481QQPPYSKCFE DHLEQKYVQL FWGLPSLHSE SLHPTVFVQH GRSSMFVFFN GITNTSMSHE
541SPVLPPPQPL FLPSTQPLPL PQTLPRGQSL HLTQVKSLAQ PQSPFPALPP SPLFLIRVCG
601VCFHRPQNEA RSLMPSEINH LEWNVLQKVQ ESVWGLPSVV QKSQEDFCPP APNPVLVRKS
661FKVHVPISII PGDFPLSSEV RKKLEQHIRK RLIQRRWGLP RRIHESLSLL RPQNKISELS
721VSESIHGPLN ISLVEGQRCN VLKKSASSFP RSFHERSSNM LSMENVGNYQ GCSQETAPKN
781HLLHDPETSS DEDLRSNSER DLGTHMMHLS GNDSGVRLGQ KQLENALTVH LSKKFEEINE
841GRMPGTVHSS WHSVKQTICL PEKSHSQIKH RNLAALVSED HGVDTSQEMS FLSSNKQKML
901EAHIKSFHMK PILNLSI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

917 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.