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SPATA20 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPATA20 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110228-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATA20 (NM_022827), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Sperm-specific protein 411 | Sperm protein SSP411 | Tisp78 | SPATA20 | Spermatogenesis associated 20 | SSP411
Origin Species
Human
NCBI GenBank
NM_022827
SwissProt
Q8TB22

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLGARAWLGR VLLLPRAGAG LAASRRCPGV WPRTWPHRSP SRGSSSRDKD RSATVSSSVP
61MPAGGKGSHP SSTPQRVPNR LIHEKSPYLL QHAYNPVDWY PWGQEAFDKA RKENKPIFLS
121VGYSTCHWCH MMEEESFQNE EIGRLLSEDF VSVKVDREER PDVDKVYMTF VQATSSGGGW
181PMNVWLTPNL QPFVGGTYFP PEDGLTRVGF RTVLLRIREQ WKQNKNTLLE NSQRVTTALL
241ARSEISVGDR QLPPSAATVN NRCFQQLDEG YDEEYGGFAE APKFPTPVIL SFLFSYWLSH
301RLTQDGSRAQ QMALHTLKMM ANGGIRDHVG QGFHRYSTDR QWHVPHFEKM LYDQAQLAVA
361YSQAFQLSGD EFYSDVAKGI LQYVARSLSH RSGGFYSAED ADSPPERGQR PKEGAYYVWT
421VKEVQQLLPE PVLGATEPLT SGQLLMKHYG LTEAGNISPS QDPKGELQGQ NVLTVRYSLE
481LTAARFGLDV EAVRTLLNSG LEKLFQARKH RPKPHLDSKM LAAWNGLMVS GYAVTGAVLG
541QDRLINYATN GAKFLKRHMF DVASGRLMRT CYTGPGGTVE HSNPPCWGFL EDYAFVVRGL
601LDLYEASQES AWLEWALRLQ DTQDKLFWDS QGGGYFCSEA ELGAGLPLRL KDDQDGAEPS
661ANSVSAHNLL RLHGFTGHKD WMDKCVCLLT AFSERMRRVP VALPEMVRAL SAQQQTLKQI
721VICGDRQAKD TKALVQCVHS VYIPNKVLIL ADGDPSSFLS RQLPFLSTLR RLEDQATAYV
781CENQACSVPI TDPCELRKLL HP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

802 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.