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SPATA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPATA2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G115574-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATA2 (NM_006038), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PD1 | KIAA0757 | SPATA2 | Tamo | Spermatogenesis associated PD1 | Spermatogenesis associated 2
Origin Species
Human
NCBI GenBank
NM_006038
SwissProt
Q9UM82

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGKPSSMDTK FKDDLFRKYV QFHESKVDTT TSRQRPGSDE CLRVAASTLL SLHKVDPFYR
61FRLIQFYEVV ESSLRSLSSS SLRALHGAFS MLETVGINLF LYPWKKEFRS IKTYTGPFVY
121YVKSTLLEED IRAILSCMGY TPELGTAYKL RELVETLQVK MVSFELFLAK VECEQMLEIH
181SQVKDKGYSE LDIVSERKSS AEDVRGCSDA LRRRAEGREH LTASMSRVAL QKSASERAAK
241DYYKPRVTKP SRSVDAYDSY WESRKPPLKA SLSLRKEPVA TDVGDDLKDE IIRPSPSLLT
301MASSPHGSPD VLPPASPSNG PALLRGTYFS TQDDVDLYTD SEPRATYRRQ DALRPDVWLL
361RNDAHSLYHK RSPPAKESAL SKCQSCGLSC SSSLCQRCDS LLTCPPASKP SAFPSKASTH
421DSLAHGASLR EKYPGQTQGL DRLPHLHSKS KPSTTPTSRC GFCNRPGATN TCTQCSKVSC
481DACLSAYHYD PCYKKSELHK FMPNNQLNYK STQLSHLVYR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

520 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.