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SPATA13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPATA13 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G105929-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPATA13 (NM_153023), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ARHGEF29 | ASEF2 | RP11-307N16.3 | Spermatogenesis associated 13 | SPATA13
Origin Species
Human
NCBI GenBank
NM_001166271
SwissProt
Q96N96

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSASPEDQN APVGCPKGAR RRRPISVIGG VSLYGTNQTE ELDNLLTQPA SRPPMPAHQV
61PPYKAVSARF RPFTFSQSTP IGLDRVGRRR QMRASNVSSD GGTEPSALVD DNGSEEDFSY
121EDLCQASPRY LQPGGEQLAI NELISDGNVV CAEALWDHVT MDDQELGFKA GDVIQVLEAS
181NKDWWWGRSE DKEAWFPASF VRLRVNQEEL SENSSSTPSE EQDEEASQSR HRHCENKQQM
241RTNVIREIMD TERVYIKHLR DICEGYIRQC RKHTGMFTVA QLATIFGNIE DIYKFQRKFL
301KDLEKQYNKE EPHLSEIGSC FLQNQEGFAI YSEYCNNHPG ACLELANLMK QGKYRHFFEA
361CRLLQQMIDI AIDGFLLTPV QKICKYPLQL AELLKYTTQE HGDYSNIKAA YEAMKNVACL
421INERKRKLES IDKIARWQVS IVGWEGLDIL DRSSELIHSG ELTKITKQGK SQQRTFFLFD
481HQLVSCKKDL LRRDMLYYKG RLDMDEMELV DLGDGRDKDC NLSVKNAFKL VSRTTDEVYL
541FCAKKQEDKA RWLQACADER RRVQEDKEMG MEISENQKKL AMLNAQKAGH GKSKGYNRCP
601VAPPHQGLHP IHQRHITMPT SVPQQQVFGL AEPKRKSSLF WHTFNRLTPF RK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

652 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.