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Spastin Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Spastin Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103331-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Spastin(SPAST) (NM_199436), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ADPSP | KIAA1083 | FSP2 | Spastin | SPAST | Spastic paraplegia 4 protein | SPG4
Origin Species
Human
NCBI GenBank
NM_014946
SwissProt
Q9UBP0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
63.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNSPGGRGKK KGSGGASNPV PPRPPPPCLA PAPPAAGPAP PPESPHKRNL YYFSYPLFVG
61FALLRLVAFH LGLLFVWLCQ RFSRALMAAK RSSGAAPAPA SASAPAPVPG GEAERVRVFH
121KQAFEYISIA LRIDEDEKAG QKEQAVEWYK KGIEELEKGI AVIVTGQGEQ CERARRLQAK
181MMTNLVMAKD RLQLLESGAV PKRKDPLTHT SNSLPRSKTV MKTGSAGLSG HHRAPSYSGL
241SMVSGVKQGS GPAPTTHKGT PKTNRTNKPS TPTTATRKKK DLKNFRNVDS NLANLIMNEI
301VDNGTAVKFD DIAGQDLAKQ ALQEIVILPS LRPELFTGLR APARGLLLFG PPGNGKTMLA
361KAVAAESNAT FFNISAASLT SKYVGEGEKL VRALFAVARE LQPSIIFIDE VDSLLCERRE
421GEHDASRRLK TEFLIEFDGV QSAGDDRVLV MGATNRPQEL DEAVLRRFIK RVYVSLPNEE
481TRLLLLKNLL CKQGSPLTQK ELAQLARMTD GYSGSDLTAL AKDAALGPIR ELKPEQVKNM
541SASEMRNIRL SDFTESLKKI KRSVSPQTLE AYIRWNKDFG DTTV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

584 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.