Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPARCL1 / Hevin Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116470-100
Catalog Number: LS-G116470
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SPARCL1 (NM_004684), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Hevin | PIG33 | SC1 | SPARCL1 | Mast9 | SPARC-like 1 (hevin) | SPARC-like 1 (mast9 | hevin) | SPARC-like protein 1 | MAST 9
- Origin Species
- Human
- NCBI GenBank
- NM_004684
- SwissProt
- Q14515
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 75 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKTGLFFLCL LGTAAAIPTN ARLLSDHSKP TAETVAPDNT AIPSLRAEDE ENEKETAVST61EDDSHHKAEK SSVLKSKEES HEQSAEQGKS SSQELGLKDQ EDSDGDLSVN LEYAPTEGTL121DIKEDMSEPQ EKKLSENTDF LAPGVSSFTD SNQQESITKR EENQEQPRNY SHHQLNRSSK181HSQGLRDQGN QEQDPNISNG EEEEEKEPGE VGTHNDNQER KTELPREHAN SKQEEDNTQS241DDILEESDQP TQVSKMQEDE FDQGNQEQED NSNAEMEEEN ASNVNKHIQE TEWQSQEGKT301GLEAISNHKE TEEKTVSEAL LMEPTDDGNT TPRNHGVDDD GDDDGDDGGT DGPRHSASDD361YFIPSQAFLE AERAQSIAYH LKIEEQREKV HENENIGTTE PGEHQEAKKA ENSSNEEETS421SEGNMRVHAV DSCMSFQCKR GHICKADQQG KPHCVCQDPV TCPPTKPLDQ VCGTDNQTYA481SSCHLFATKC RLEGTKKGHQ LQLDYFGACK SIPTCTDFEV IQFPLRMRDW LKNILMQLYE541ANSEHAGYLN EKQRNKVKKI YLDEKRLLAG DHPIDLLLRD FKKNYHMYVY PVHWQFSELD601QHPMDRVLTH SELAPLRASL VPMEHCITRF FEECDPNKDK HITLKEWGHC FGIKEEDIDE661NLLFTRTRPLEQKLISEEDLAANDILDYKDDDDKV
664 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.