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SPAG9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SPAG9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SPAG9 antibodies →
$725.00each
Catalog Number: LS-G116975-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human JLP(SPAG9) (NM_003971), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Cancer/testis antigen 89 | CT89 | HSS | JNK interacting protein | JNK/SAPK-associated protein | JIP-4 | JIP4 | JLP | JNK-interacting protein 4 | KIAA0516 | MAPK8IP4 | HLC-6 | SPAG9 | Sperm-associated antigen 9 | Sperm-specific protein | Sperm associated antigen 9 | Lung cancer oncogene 4 | SYD1 | Sunday driver 1 | Max-binding protein | PHET | Proliferation-inducing gene 6 | HLC4 | HLC6 | PIG6 | Sperm surface protein
Origin Species
Human
NCBI GenBank
NM_003971
SwissProt
O60271

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
144.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MELEDGVVYQ EEPGGSGAVM SERVSGLAGS IYREFERLIG RYDEEVVKEL MPLVVAVLEN
61LDSVFAQDQE HQVELELLRD DNEQLITQYE REKALRKHAE EKFIEFEDSQ EQEKKDLQTR
121VESLESQTRQ LELKAKNYAD QISRLEEREA ELKKEYNALH QRHTEMIHNY MEHLERTKLH
181QLSGSDQLES TAHSRIRKER PISLGIFPLP AGDGLLTPDA QKGGETPGSE QWKFQELSQP
241RSHTSLKDEL SDVSQGGSKA TTPASTANSD VATIPTDTPL KEENEGFVKV TDAPNKSEIS
301KHIEVQVAQE TRNVSTGSAE NEEKSEVQAI IESTPELDMD KDLSGYKGSS TPTKGIENKA
361FDRNTESLFE ELSSAGSGLI GDVDEGADLL GMGREVENLI LENTQLLETK NALNIVKNDL
421IAKVDELTCE KDVLQGELEA VKQAKLKLEE KNRELEEELR KARAEAEDAR QKAKDDDDSD
481IPTAQRKRFT RVEMARVLME RNQYKERLME LQEAVRWTEM IRASRENPAM QEKKRSSIWQ
541FFSRLFSSSS NTTKKPEPPV NLKYNAPTSH VTPSVKKRSS TLSQLPGDKS KAFDFLSEET
601EASLASRREQ KREQYRQVKA HVQKEDGRVQ AFGWSLPQKY KQVTNGQGEN KMKNLPVPVY
661LRPLDEKDTS MKLWCAVGVN LSGGKTRDGG SVVGASVFYK DVAGLDTEGS KQRSASQSSL
721DKLDQELKEQ QKELKNQEEL SSLVWICTST HSATKVLIID AVQPGNILDS FTVCNSHVLC
781IASVPGARET DYPAGEDLSE SGQVDKASLC GSMTSNSSAE TDSLLGGITV VGCSAEGVTG
841AATSPSTNGA SPVMDKPPEM EAENSEVDEN VPTAEEATEA TEGNAGSAED TVDISQTGVY
901TEHVFTDPLG VQIPEDLSPV YQSSNDSDAY KDQISVLPNE QDLVREEAQK MSSLLPTMWL
961GAQNGCLYVH SSVAQWRKCL HSIKLKDSIL SIVHVKGIVL VALADGTLAI FHRGVDGQWD
1021LSNYHLLDLG RPHHSIRCMT VVHDKVWCGY RNKIYVVQPK AMKIEKSFDA HPRKESQVRQ
1081LAWVGDGVWV SIRLDSTLRL YHAHTYQHLQ DVDIEPYVSK MLGTGKLGFS FVRITALMVS
1141CNRLWVGTGN GVIISIPLTE TNKTSGVPGN RPGSVIRVYG DENSDKVTPG TFIPYCSMAH
1201AQLCFHGHRD AVKFFVAVPG QVISPQSSSS GTDLTGDKAG PSAQEPGSQT PLKSMLVISG
1261GEGYIDFRMG DEGGESELLG EDLPLEPSVT KAERSHLIVW QVMYGNE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1307 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.