Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SPAG5 / MAP126 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G100825-100
Catalog Number: LS-G100825
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MAP126(SPAG5) (NM_006461), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Astrin | DEEPEST | MAP126 | Sperm associated antigen 5 | Sperm-associated antigen 5 | HMAP126 | SPAG5
- Origin Species
- Human
- NCBI GenBank
- NM_006461
- SwissProt
- Q96R06
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 134.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MWRVKKLSLS LSPSPQTGKP SMRTPLRELT LQPGALTNSG KRSPACSSLT PSLCKLGLQE61GSNNSSPVDF VNNKRTDLSS EHFSHSSKWL ETCQHESDEQ PLDPIPQISS TPKTSEEAVD121PLGNYMVKTI VLVPSPLGQQ QDMIFEARLD TMAETNSISL NGPLRTDDLV REEVAPCMGD181RFSEVAAVSE KPIFQESPSH LLEESPPNPC SEQLHCSKES LSSRTEAVRE DLVPSESNAF241LPSSVLWLSP STALAADFRV NHVDPEEEIV EHGAMEEREM RFPTHPKESE TEDQALVSSV301EDILSTCLTP NLVEMESQEA PGPAVEDVGR ILGSDTESWM SPLAWLEKGV NTSVMLENLR361QSLSLPSMLR DAAIGTTPFS TCSVGTWFTP SAPQEKSTNT SQTGLVGTKH STSETEQLLC421GRPPDLTALS RHDLEDNLLS SLVILEVLSR QLRDWKSQLA VPHPETQDSS TQTDTSHSGI481TNKLQHLKES HEMGQALQQA RNVMQSWVLI SKELISLLHL SLLHLEEDKT TVSQESRRAE541TLVCCCFDLL KKLRAKLQSL KAEREEARHR EEMALRGKDA AEIVLEAFCA HASQRISQLE601QDLASMREFR GLLKDAQTQL VGLHAKQEEL VQQTVSLTST LQQDWRSMQL DYTTWTALLS661RSRQLTEKLT VKSQQALQER DVAIEEKQEV SRVLEQVSAQ LEECKGQTEQ LELENSRLAT721DLRAQLQILA NMDSQLKELQ SQHTHCAQDL AMKDELLCQL TQSNEEQAAQ WQKEEMALKH781MQAELQQQQA VLAKEVRDLK ETLEFADQEN QVAHLELGQV ECQLKTTLEV LRERSLQCEN841LKDTVENLTA KLASTIADNQ EQDLEKTRQY SQKLGLLTEQ LQSLTLFLQT KLKEKTEQET901LLLSTACPPT QEHPLPNDRT FLGSILTAVA DEEPESTPVP LLGSDKSAFT RVASMVSLQP961AETPGMEESL AEMSIMTTEL QSLCSLLQES KEEAIRTLQR KICELQARLQ AQEEQHQEVQ1021KAKEADIEKL NQALCLRYKN EKELQEVIQQ QNEKILEQID KSGELISLRE EVTHLTRSLR1081RAETETKVLQ EALAGQLDSN CQPMATNWIQ EKVWLSQEVD KLRVMFLEMK NEKEKLMIKF1141QSHRNILEEN LRRSDKELEK LDDIVQHIYK TLLSIPEVVR GCKELQGLLE FLSTRTRPLE1201QKLISEEDLA ANDILDYKDD DDKV
1224 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.