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SP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SP9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126590-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Transcription Factor SP9(SP9) (NM_001145250), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SP9 | Zinc finger protein 990 | ZNF990 | CRCRSP33 | Sp9 transcription factor | Transcription factor Sp9
Origin Species
Human
SwissProt
P0CG40

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MATSILGEEP RFGTTPLAML AATCNKIGNT SPLTTLPESS AFAKGGFHPW KRSSSSCNLG
61SSLSGFAVAT GGRGSGGLAG GSGAANSAFC LASTSPTSSA FSSDYGGLFS NSAAAAAAAA
121GVSPQEAGGQ SAFISKVHTT AADGLYPRVG MAHPYESWYK SGFHSTLAAG EVTNGAASSW
181WDVHSSPGSW LEVQNPAGGL QSSLHSGAPQ ASLHSQLGTY NPDFSSLTHS AFSSTGLGSS
241AAAASHLLST SQHLLAQDGF KPVLPSYSDS SAAVAAAAAS AMISGAAAAA AGGSSARSAR
301RYSGRATCDC PNCQEAERLG PAGASLRRKG LHSCHIPGCG KVYGKTSHLK AHLRWHTGER
361PFVCNWLFCG KRFTRSDELQ RHLRTHTGEK RFACPVCNKR FMRSDHLSKH IKTHNGGGGG
421KKGSDSDTDA SNLETPRSES PDLILHDSGV SAARAAAAAA AAAAAAAAAA SAGGKEAASG
481PNDS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

484 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.