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SP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SP8 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104179-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SP8 (NM_182700), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SP8 | Specificity protein 8 | Transcription factor Sp8 | Sp8 transcription factor
Origin Species
Human
SwissProt
Q8IXZ3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
50.3 kda
Protein Type
Lysate
Amino Acid Sequence
1MATSLLGEEP RLGSTPLAML AATCNKIGSP SPSPSSLSDS SSSFGKGFHP WKRSSSSSSA
61SCNVVGSSLS SFGVSGASRN GGSSSAAAAA AAAAAAAAAL VSDSFSCGGS PGSSAFSLTS
121SSAAAAAAAA AAAASSSPFA NDYSVFQAPG VSGGSGGGGG GGGGGSSAHS QDGSHQPVFI
181SKVHTSVDGL QGIYPRVGMA HPYESWFKPS HPGLGAAGEV GSAGASSWWD VGAGWIDVQN
241PNSAAALPGS LHPAAGGLQT SLHSPLGGYN SDYSGLSHSA FSSGASSHLL SPAGQHLMDG
301FKPVLPGSYP DSAPSPLAGA GGSMLSAGPS APLGGSPRSS ARRYSGRATC DCPNCQEAER
361LGPAGASLRR KGLHSCHIPG CGKVYGKTSH LKAHLRWHTG ERPFVCNWLF CGKRFTRSDE
421LQRHLRTHTG EKRFACPVCN KRFMRSDHLS KHVKTHSGGG GGGGSAGSGS GGKKGSDTDS
481EHSAAGSPPC HSPELLQPPE PGHRNGLE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

508 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.