Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SP32 / ACRBP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G102048-100
Catalog Number: LS-G102048
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ACRBP (NM_032489), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Acrosin binding protein | ACRBP | Cancer/testis antigen 23 | CT23 | Acrosin-binding protein | OY-TES-1 | SP32 | Cancer/testis antigen OY-TES-1
- Origin Species
- Human
- NCBI GenBank
- NM_032489
- SwissProt
- Q8NEB7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRKPAAGFLP SLLKVLLLPL APAAAQDSTQ ASTPGSPLSP TEYERFFALL TPTWKAETTC61RLRATHGCRN PTLVQLDQYE NHGLVPDGAV CSNLPYASWF ESFCQFTHYR CSNHVYYAKR121VLCSQPVSIL SPNTLKEIEA SAEVSPTTMT SPISPHFTVT ERQTFQPWPE RLSNNVEELL181QSSLSLGGQE QAPEHKQEQG VEHRQEPTQE HKQEEGQKQE EQEEEQEEEG KQEEGQGTKE241GREAVSQLQT DSEPKFHSES LSSNPSSFAP RVREVESTPM IMENIQELIR SAQEIDEMNE301IYDENSYWRN QNPGSLLQLP HTEALLVLCY SIVENTCIIT PTAKAWKYME EEILGFGKSV361CDSLGRRHMS TCALCDFCSL KLEQCHSEAS LQRQQCDTSH KTPFVSPLLA SQSLSIGNQV421GSPESGRFYG LDLYGGLHMD FWCARLATKG CEDVRVSGWL QTEFLSFQDG DFPTKICDTD481YIQYPNYCSF KSQQCLMRNR NRKVSRMRCL QNETYSALSP GKSEDVVLRW SQEFSTLTLG541QFGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
543 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.