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SP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G99279-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SP3(SP3) (NM_001017371), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SP3 | SPR2 | Transcription factor Sp3 | Sp3 transcription factor | SPR-2 | Specificity protein 3
NCBI GenBank
X68560
SwissProt
Q02447

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
75.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGPPSPGDDE EEAAAAAGAP AAAGATGDLA SAQLGGAPNR WEVLSATPTT IKDEAGNLVQ
61IPSAATSSGQ YVLPLQNLQN QQIFSVAPGS DSSNGAVSSV QYQVIPQIQS ADGQQVQIGF
121TGSSDNGGIN QESSQIQIIP GSNQTLLASG TPSANIQNLI PQTGQVQVQG VAIGGSSFPG
181QTQVVANVPL GLPGNITFVP INSVDLDSLG LSGSSQTMTA GINADGHLIN TGQAMDSSDN
241SERTGERVSP DINETNTDTD LFVPTSSSSQ LPVTIDSTGI LQQNTNSLTT SSGQVHSSDL
301QGNYIQSPVS EETQAQNIQV STAQPVVQHL QLQESQQPTS QAQIVQGITP QTIHGVQASG
361QNISQQALQN LQLQLNPGTF LIQAQTVTPS GQVTWQTFQV QGVQNLQNLQ IQNTAAQQIT
421LTPVQTLTLG QVAAGGAFTS TPVSLSTGQL PNLQTVTVNS IDSAGIQLHP GENADSPADI
481RIKEEEPDPE EWQLSGDSTL NTNDLTHLRV QVVDEEGDQQ HQEGKRLRRV ACTCPNCKEG
541GGRGTNLGKK KQHICHIPGC GKVYGKTSHL RAHLRWHSGE RPFVCNWMYC GKRFTRSDEL
601QRHRRTHTGE KKFVCPECSK RFMRSDHLAK HIKTHQNKKG IHSSSTVLAS VEAARDDTLI
661TAGGTTLILA NIQQGSVSGI GTVNTSATSN QDILTNTEIP LQLVTVSGNE TME
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

713 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.