Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SOWAHB / ANKRD56 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120800-100
Catalog Number: LS-G120800
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD56(SOWAHB) (NM_001029870), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ANKRD56 | Ankyrin repeat domain 56 | Protein sosondowah homolog B | SOWAHB
- Origin Species
- Human
- NCBI GenBank
- NM_001029870
- SwissProt
- A6NEL2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 85.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MARELSQEAL LDFLCQAGGR VTNAALLSHF KSFLRDPDAS PSQHQHRREL FKGFVNSVAA61VRQDPDGTKY VVLKRRYRDL LGEEGLQRPR EPPAAAPSAG GAAPCSPRGA RRGEPPQQQP121RRRRREKEPE EEPAGAAARA ADAACNGLPG SDSRRAPGKG GGSKGSPGQR PPVPAAAAAG181AQARASCAAA KTQGRCCWEC LQNNLAVLPG ELGALPHSAT AEEKPARALP AQDDRGASRE241REEGALAEPA PVPAVAHSPP ATVEAATSRA SPPALLPGPA PRGDRPELLT PSSLHYSTLQ301QQQQRTREWV ARHPQVPEAR DQGPIRAWSV LPDNFLQLPL EPGSTEPNSE PPDPCLSSHS361LFPVVPDESW ESWAGNPSLT VFRSIRCQLS LQDLDDFVDQ ESDGSEESSS GPKDSPGASE421EGLQVVLGTP DRGKLRNPAG GLSVSRKEGS PSRSPQGLRN RGDGHISQQV PAGANGLAGH481PLKPLPWPVP KLRRSLRRSS LAGRAKLSSS DEEYLDEGLL KRSRRPPRSR KPSKAGTAPS541PRVDAGLSLK LAEVKAVVAE RGWRHSLWVP SGEGSAALAP HRTSEHKSSL VPLDAREHEW601IVKLASGSWI QVWTLFWEDP QLALHKDFLT GYTALHWIAK HGDLRALQDL VSGAKKAGIV661LDVNVRSSCG YTPLHLAAIH GHQGVIKLLV QRLASRVNVR DSSGKKPWQY LTSNTSGEIW721QLLGAPRGKP IFPVYPLVGS SSPTRKAKSK EISRSVTRKT SFAALLKSQH NKWKLANQYE781KFHSPREREE YSDSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
793 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.