Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SOWAHA / ANKRD43 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104935-100
Catalog Number: LS-G104935
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ANKRD43(SOWAHA) (NM_175873), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_175873
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALAAAAAAA AAGVSQAAVL GFLQEHGGKV RNSELLSRFK PLLDAGDPRG RAARRDRFKQ61FVNNVAVVKE LDGVKFVVLR KKPRPPEPEP APFGPPGAAA QPSKPTSTVL PRSASAPGAP121PLVRVPRPVE PPGDLGLPTE PQDTPGGPAS EPAQPPGERS ADPPLPALEL AQATERPSAD181AAPPPRAPSE AASPCSDPPD AEPGPGAAKG PPQQKPCMLP VRCVPAPATL RLRAEEPGLR241RQLSEEPSPR SSPLLLRRLS VEESGLGLGL GPGRSPHLRR LSRAGPRLLS PDAEELPAAP301PPSAVPLEPS EHEWLVRTAG GRWTHQLHGL LLRDRGLAAK RDFMSGFTAL HWAAKSGDGE361MALQLVEVAR RSGAPVDVNA RSHGGYTPLH LAALHGHEDA AVLLVVRLGA QVHVRDHSGR421RAYQYLRPGS SYALRRLLGD PGLRGTTEPD ATGGGSGSLA ARRPVQVAAT ILSSTTSAFL481GVLADDLMLQ DLARGLKKSS SFSKFLSASP MAPRKKTKIR GGLPAFSEIS RRPTPGPLAG541LVPSFPPTTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
549 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.