Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SORBS2 / ARGBP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126798-100
Catalog Number: LS-G126798
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SORBS2 (NM_001145675), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Arg binding protein 2 | Arg/Abl-interacting protein 2 | ARGBP2 | KIAA0777 | SORBS2 | PRO0618 | Sorbin
- Origin Species
- Human
- NCBI GenBank
- NM_021069
- SwissProt
- O94875
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 55.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKATTPLQTV DRPKDWYKTM FKQIHMVHKP DDDTDMYNTP YTYNAGLYNP PYSAQSHPAA61KTQTYRPLSK SHSDNSPNAF KDASSPVPPP HVPPPVPPLR PRDRSSTEKH DWDPPDRKVD121TRKFRSEPRS IFEYEPGKSS ILQHERPASL YQSSIDRSLE RPMSSASMAS DFRKRRKSEP181AVGPPRGLGD QSASRTSPGR VDLPGSSTTL TKSFTSSSPS SPSRAKDRES PRSYSSTLTD241MGRSAPRERR GTPEKEKLPA KAVYDFKAQT SKELSFKKGD TVYILRKIDQ NWYEGEHHGR301VGIFPISYVE KLTPPEKAQP ARPPPPAQPG EIGEAIAKYN FNADTNVELS LRKGDRVILL361KRVDQNWYEG KIPGTNRQGI FPVSYVEVVK KNTKGAEDYP DPPIPHSYSS DRIHSLSSNK421PQRPVFTHEN IQGGGEPFQA LYNYTPRNED ELELRESDVI DVMEKCDDGW FVGTSRRTKF481FGTFPGNYVK RLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
492 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.