Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SOLO / SESTD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104778-100
Catalog Number: LS-G104778
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Solo(SESTD1) (NM_178123), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Protein Solo | SEC14 and spectrin domains 1 | SESTD1 | SOLO
- Origin Species
- Human
- SwissProt
- Q86VW0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEASVILPIL KKKLAFLSGG KDRRSGLILT IPLCLEQTNM DELSVTLDFL LSIPSEKCKA61RGFTVIVDGR KSQWNVVKTV VVMLQNVVPA EVSLVCVVKP DEFWDKKVTH FCFWKEKDRL121GFEVILVSAN KLTRYIEPCQ LTEDFGGSLT YDHMDWLNKR LVFEKFTKES TSLLDELALI181NNGSDKGNQQ EKERSVDLNF LPSVDPETVL QTGHELLSEL QQRRFNGSDG GVSWSPMDDE241LLAQPQVMKL LDSLREQYTR YQEVCRQRSK RTQLEEIQQK VMQVVNWLEG PGSEQLRAQW301GIGDSIRASQ ALQQKHEEIE SQHSEWFAVY VELNQQIAAL LNAGDEEDLV ELKSLQQQLS361DVCYRQASQL EFRQNLLQAA LEFHGVAQDL SQQLDGLLGM LCVDVAPADG ASIQQTLKLL421EEKLKSVDVG LQGLREKGQG LLDQISNQAS WAYGKDVTIE NKENVDHIQG VMEDMQLRKQ481RCEDMVDVRR LKMLQMVQLF KCEEDAAQAV EWLSELLDAL LKTHIRLGDD AQETKVLLEK541HRKFVDVAQS TYDYGRQLLQ ATVVLCQSLR CTSRSSGDTL PRLNRVWKQF TIASEERVHR601LEMAIAFHSN AEKILQDCPE EPEAINDEEQ FDEIEAVGKS LLDRLTVPVV YPDGTEQYFG661SPSDMASTAE NIRDRMKLVN LKRQQLRHPE MVTTESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
696 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.