Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SOGA3 / C6orf174 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G121823-100
Catalog Number: LS-G121823
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C6orf174(SOGA3) (NM_001012279), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DJ403A15.3 | Protein SOGA3 | SOGA3 | C6orf174 | SOGA family member 3
- Origin Species
- Human
- NCBI GenBank
- NM_001012279
- SwissProt
- Q5TF21
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 103 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSQPPIGGAA PATAAASPAA AATEARLHPE GSSRKQQRAQ SPARPRDSSL RQTIAATRSP61VGAGTKLNSV RQQQLQQQQQ QGNKTGSRTG PPASIRGGGG GAEKATPLAP KGAAPGAVQP121VAGAEAAPAA TLAALGGRRP GPPEEPPREL ESVPSKLGEP PPLGEGGGGG GEGGGAGGGS181GEREGGAPQP PPPRGWRGKG VRAQQRGGSG GEGASPSPSS SSAGKTPGTG SRNSGSGVAG241GGSGGGGSYW KEGCLQSELI QFHLKKERAA AAAAAAQMHA KNGGGSSSRS SPVSGPPAVC301ETLAVASASP MAAAAEGPQQ SAEGSASGGG MQAAAPPSSQ PHPQQLQEQE EMQEEMEKLR361EENETLKNEI DELRTEMDEM RDTFFEEDAC QLQEMRHELE RANKNCRILQ YRLRKAERKR421LRYAQTGEID GELLRSLEQD LKVAKDVSVR LHHELENVEE KRTTTEDENE KLRQQLIEVE481IAKQALQNEL EKMKELSLKR RGSKDLPKSE KKAQQTPTEE DNEDLKCQLQ FVKEEAALMR541KKMAKIDKEK DRFEHELQKY RSFYGDLDSP LPKGEAGGPP STREAELKLR LRLVEEEANI601LGRKIVELEV ENRGLKAELD DLRGDDFNGS ANPLMREQSE SLSELRQHLQ LVEDETELLR661RNVADLEEQN KRITAELNKY KYKSGGHDSA RHHDNAKTEA LQEELKAARL QINELSGKVM721QLQYENRVLM SNMQRYDLAS HLGIRGSPRD SDAESDAGKK ESDDDSRPPH RKREGPIGGE781SDSEEVRNIR CLTPTRSFYP APGPWPKSFS DRQQMKDIRS EAERLGKTID RLIADTSTII841TEARIYVANG DLFGLMDEED DGSRIREHEL LYRINAQMKA FRKELQTFID RLEVPKSADD901RGAEEPISVS QMFQPIILLI LILVLFSSLS YTTIFKLVFL FTLFFVLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
947 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.