Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SOCS7 / SOCS-7 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113843-100
Catalog Number: LS-G113843
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SOCS7 (NM_014598), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- NAP-4 | Nck-associated protein 4 | SOCS7 | SOCS-7 | NAP4 | NCKAP4
- Origin Species
- Human
- NCBI GenBank
- AB005216
- SwissProt
- O14512
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.8 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MVFRNVGRPP EEEDVEAAPE PGPSELLCPR HRCALDPKAL PPGLALERTW GPAAGLEAQL61AALGLGQPAG PGVKTVGGGC CPCPCPPQPP PPQPQPPAAA PQAGEDPTET SDALLVLEGL121ESEAESLETN SCSEEELSSP GRGGGGGGRL LLQPPGPELP PVPFPLQDLV PLGRLSRGEQ181QQQQQQQPPP PPPPPGPLRP LAGPSRKGSF KIRLSRLFRT KSCNGGSGGG DGTGKRPSGE241LAASAASLTD MGGSAGRELD AGRKPKLTRT QSAFSPVSFS PLFTGETVSL VDVDISQRGL301TSPHPPTPPP PPRRSLSLLD DISGTLPTSV LVAPMGSSLQ SFPLPPPPPP HAPDAFPRIA361PIRAAESLHS QPPQHLQCPL YRPDSSSFAA SLRELEKCGW YWGPMNWEDA EMKLKGKPDG421SFLVRDSSDP RYILSLSFRS QGITHHTRME HYRGTFSLWC HPKFEDRCQS VVEFIKRAIM481HSKNGKFLYF LRSRVPGLPP TPVQLLYPVS RFSNVKSLQH LCRFRIRQLV RIDHIPDLPL541PKPLISYIRK FYYYDPQEEV YLSLKEAQLI SKQKQEVEPS TTRTRPLEQKLISEEDLAANDILDYKDDDDKV
581 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.