LSBio
SNX8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SNX8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G101135-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SNX8 (NM_013321), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Sorting nexin-8 | Mvp1 | SNX8 | Sorting nexin 8
Origin Species
Human
NCBI GenBank
NM_013321
SwissProt
Q9Y5X2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MTGRAMDPLP AAAVGAAAEA EADEEADPPA SDLPTPQAIE PQAIVQQVPA PSRMQMPQGN
61PLLLSHTLQE LLARDTVQVE LIPEKKGLFL KHVEYEVSSQ RFKSSVYRRY NDFVVFQEML
121LHKFPYRMVP ALPPKRMLGA DREFIEARRR ALKRFVNLVA RHPLFSEDVV LKLFLSFSGS
181DVQNKLKESA QCVGDEFLNC KLATRAKDFL PADIQAQFAI SRELIRNIYN SFHKLRDRAE
241RIASRAIDNA ADLLIFGKEL SAIGSDTTPL PSWAALNSST WGSLKQALKG LSVEFALLAD
301KAAQQGKQEE NDVVEKLNLF LDLLQSYKDL CERHEKGVLH KHQRALHKYS LMKRQMMSAT
361AQNREPESVE QLESRIVEQE NAIQTMELRN YFSLYCLHQE TQLIHVYLPL TSHILRAFVN
421SQIQGHKEMS KVWNDLRPKL SCLFAGPHST LTPPCSPPED GLCPH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

465 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.