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SNX33 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SNX33 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SNX33 antibodies →
$504.00each
Catalog Number: LS-G105853-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SNX33 (NM_153271), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SH3PXD3C | Sorting nexin 33 | Sorting nexin-33 | SH3 and PX domain containing 3 | SH3PX3 | SNX33
Origin Species
Human
NCBI GenBank
NM_153271
SwissProt
Q8WV41

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALKGRALYD FHSENKEEIS IQQDEDLVIF SETSLDGWLQ GQNSRGETGL FPASYVEIVR
61SGISTNHADY SSSPAGSPGA QVSLYNSPSV ASPARSGGGS GFLSNQGSFE EDDDDDWDDW
121DDGCTVVEEP RAGGLGTNGH PPLNLSYPGA YPSQHMAFRP KPPLERQDSL ASAKRGSVVG
181RNLNRFSCFV RSGVEAFILG DVPMMAKIAE TYSIEMGPRG PQWKANPHPF ACSVEDPTKQ
241TKFKGIKSYI SYKLTPTHAA SPVYRRYKHF DWLYNRLLHK FTVISVPHLP EKQATGRFEE
301DFIEKRKRRL ILWMDHMTSH PVLSQYEGFQ HFLSCLDDKQ WKMGKRRAEK DEMVGASFLL
361TFQIPTEHQD LQDVEDRVDT FKAFSKKMDD SVLQLSTVAS ELVRKHVGGF RKEFQKLGSA
421FQAISHSFQM DPPFCSEALN SAISHTGRTY EAIGEMFAEQ PKNDLFQMLD TLSLYQGLLS
481NFPDIIHLQK GAFAKVKESQ RMSDEGRMVQ DEADGIRRRC RVVGFALQAE MNHFHQRREL
541DFKHMMQNYL RQQILFYQRV GQQLEKTLRM YDNL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

574 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.