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SNX21 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SNX21 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SNX21 antibodies →
$504.00each
Catalog Number: LS-G105987-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SNX21 (NM_152897), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C20orf161 | PP3993 | Sorting nexin 21 | Sorting nexin family member 21 | Sorting nexin-21 | SNX-L | DJ337O18.4 | SNX21 | SNXL | Sorting nexin L
Origin Species
Human
NCBI GenBank
AF258570
SwissProt
Q969T3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
21.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MHRGTQEGAM ASRLLHRLRH ALAGDGPGEA AASPEAEQFP ESSELEDDDA EGLSSRLSGT
61LSFTSAEDDE DDEDEDDEEA GPDQLPLGDG TSGEDAERSP PPDGQWGSQL LARQLQDFWK
121KSRNTLAPQR LLFEVTSANV VKDPPSKYVL YTLTVIGPGP PDCQPAQISR RYSDFERLHR
181NLQRQFRGPM AAISFPQSH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

199 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.