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SNX19 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SNX19 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SNX19 antibodies →
$725.00each
Catalog Number: LS-G113723-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SNX19 (NM_014758), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CHET8 | KIAA0254 | Sorting nexin-19 | SNX19 | Sorting nexin 19
Origin Species
Human
NCBI GenBank
AL355516
SwissProt
Q92543

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKTETVPPFQ ETPAGSSCHL NNLLSSRKLM AVGVLLGWLL VIHLLVNVWL LCLLSALLVV
61LGGWLGSSLA GVASGRLHLE RFIPLATCPP CPEAERQLER EINRTIQMII RDFVLSWYRS
121VSQEPAFEEE MEAAMKGLVQ ELRRRMSVMD SHAVAQSVLT LCGCHLQSYI QAKEATAGKN
181GPVEPSHLWE AYCRATAPHP AVHSPSAEVT YTRGVVNLLL QGLVPKPHLE TRTGRHVVVE
241LITCNVILPL ISRLSDPDWI HLVLVGIFSK ARDPAPCPAS APEQPSVPTS LPLIAEVEQL
301PEGRASPVAA PVFLSYSEPE GSAGPSPEVE EGHEAVEGDL GGMCEERKVG NNSSHFLQPN
361VRGPLFLCED SELESPLSEL GKETIMLMTP GSFLSDRIQD ALCALESSQA LEPKDGEASE
421GAEAEEGPGT ETETGLPVST LNSCPEIHID TADKEIEQGD VTASVTALLE GPEKTCPSRP
481SCLEKDLTND VSSLDPTLPP VLLSSSPPGP LSSATFSFEP LSSPDGPVII QNLRITGTIT
541AREHSGTGFH PYTLYTVKYE TALDGENSSG LQQLAYHTVN RRYREFLNLQ TRLEEKPDLR
601KFIKNVKGPK KLFPDLPFGN MDSDRVEARK SLLESFLKQL CAIPEIANSE EVQEFLALNT
661DARIAFVKKP FMVSRIDKMV VSAIVDTLKT AFPRSEPQSP TEELSEAETE SKPQTEGKKA
721SKSRLRFSSS KISPALSVTE AQDKILYCLQ EGNVESETLS MSAMESFIEK QTKLLEMQPT
781KAPEKDPEQP PKGRVDSCVS DAAVPAQDPS NSDPGTETEL ADTALDLLLL LLTEQWKWLC
841TENMQKFLRL IFGTLVQRWL EVQVANLTSP QRWVQYLLLL QESIWPGGVL PKFPRPVRTQ
901EQKLAAEKQA LQSLMGVLPD LVVEILGVNK CRLSWGLVLE SLQQPLINRH LIYCLGDIIL
961EFLDLSASVE ESAATTSASD TPGNSKRMGV SSSGP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

995 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.