Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SNTG2 / SYN5 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G111536-100
Catalog Number: LS-G111536
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Syntrophin gamma 2(SNTG2) (NM_018968), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- G2SYN | Gamma-2-syntrophin | Syntrophin-5 | SYN5 | Syntrophin | gamma 2 | SNTG2
- Origin Species
- Human
- SwissProt
- Q9NY99
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MGTEGPPPPA ASRGRQGCLL VPARTKTTIA LLYDEESENA YDIRLKLTKE VLTIQKQDVV61CVGGSHQGRN RRTVTLRRQP VGGLGLSIKG GSEHNVPVVI SKIFEDQAAD QTGMLFVGDA121VLQVNGIHVE NATHEEVVHL LRNAGDEVTI TVEYLREAPA FLKLPLGSPG PSSDHSSGAS181SPLFDSGLHL NGNSSTTAPS SPSSPIAKDP RYEKRWLDTL SVPLSMARIS RYKAGTEKLR241WNAFEVLALD GVSSGILRFY TAQDGTDWLR AVSANIRELT LQNMKMANKC CSPSDQVVHM301GWVNEKLQGA DSSQTFRPKF LALKGPSFYV FSTPPVSTFD WVRAERTYHL CEVLFKVHKF361WLTEDCWLQA NLYLGLQDFD FEDQRPYCFS IVAGHGKSHV FNVELGSELA MWEKSFQRAT421FMEVQRTGSR TYMCSWQGEM LCFTVDFALG FTCFESKTKN VLWRFKFSQL KGSSDDGKTR481VKLLFQNLDT KQIETKELEF QDLRAVLHCI HSFIAAKVAS VDPGFMDSQS LARKYMYSSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
539 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.