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SNED1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SNED1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SNED1 antibodies →
$725.00each
Catalog Number: LS-G120223-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SNED1 (NM_001080437), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
6720455I24Rik homolog | IRE-BP1 | SNED1 | Snep | SST3
Origin Species
Human
NCBI GenBank
NM_001080437
SwissProt
Q8TER0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
152 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRHGVAWALL VAAALGLGAR GVRGAVALAD FYPFGAERGD AVTPKQDDGG SGLRPLSVPF
61PFFGAEHSGL YVNNNGIISF LKEVSQFTPV AFPIAKDRCV VAAFWADVDN RRAGDVYYRE
121ATDPAMLRRA TEDVRHYFPE LLDFNATWVF VATWYRVTFF GGSSSSPVNT FQTVLITDGK
181LSFTIFNYES IVWTTGTHAS SGGNATGLGG IAAQAGFNAG DGQRYFSIPG SRTADMAEVE
241TTTNVGVPGR WAFRIDDAQV RVGGCGHTTS VCLALRPCLN GGKCIDDCVT GNPSYTCSCL
301SGFTGRRCHL DVNECASQPC QNGGTCTHGI NSFRCQCPAG FGGPTCETAQ SPCDTKECQH
361GGQCQVENGS AVCVCQAGYT GAACEMDVDD CSPDPCLNGG SCVDLVGNYT CLCAEPFKGL
421RCETGDHPVP DACLSAPCHN GGTCVDADQG YVCECPEGFM GLDCRERVPD DCECRNGGRC
481LGANTTLCQC PLGFFGLLCE FEITAMPCNM NTQCPDGGYC MEHGGSYLCV CHTDHNASHS
541LPSPCDSDPC FNGGSCDAHD DSYTCECPRG FHGKHCEKAR PHLCSSGPCR NGGTCKEAGG
601EYHCSCPYRF TGRHCEIGKP DSCASGPCHN GGTCFHYIGK YKCDCPPGFS GRHCEIAPSP
661CFRSPCVNGG TCEDRDTDFF CHCQAGYMGR RCQAEVDCGP PEEVKHATLR FNGTRLGAVA
721LYACDRGYSL SAPSRIRVCQ PHGVWSEPPQ CLEIDECRSQ PCLHGGSCQD RVAGYLCLCS
781TGYEGAHCEL ERDECRAHPC RNGGSCRNLP GAYVCRCPAG FVGVHCETEV DACDSSPCQH
841GGRCESGGGA YLCVCPESFF GYHCETVSDP CFSSPCGGRG YCLASNGSHS CTCKVGYTGE
901DCAKELFPPT ALKMERVEES GVSISWNPPN GPAARQMLDG YAVTYVSSDG SYRRTDFVDR
961TRSSHQLQAL AAGRAYNISV FSVKRNSNNK NDISRPAVLL ARTRPRPVEG FEVTNVTAST
1021ISVQWALHRI RHATVSGVRV SIRHPEALRD QATDVDRSVD RFTFRALLPG KRYTIQLTTL
1081SGLRGEEHPT ESLATAPTHV WTRPLPPANL TAARVTATSA HVVWDAPTPG SLLEAYVINV
1141TTSQSTKSRY VPNGKLASYT VRDLLPGRRY QLSVIAVQST ELGPQHSEPA HLYIITSPRD
1201GADRRWHQGG HHPRVLKNRP PPARLPELRL LNDHSAPETP TQPPRFSELV DGRGRVSARF
1261GGSPSKAATV RSQPTASAQL ENMEEAPKRV SLALQLPEHG SKDIGNVPGN CSENPCQNGG
1321TCVPGADAHS CDCGPGFKGR RCELACIKVS RPCTRLFSET KAFPVWEGGV CHHVYKRVYR
1381VHQDICFKES CESTSLKKTP NRKQSKSQTL EKS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1413 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.