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SNAPC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SNAPC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G120450-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SNAPC3 (NM_001039697), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PTF subunit beta | SNAPC3 | SNAP50 | SNAPc 50 kDa subunit | SNAPc subunit 3 | PTFbeta
Origin Species
Human
NCBI GenBank
U66413
SwissProt
Q92966

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
46.6 kda
Protein Type
Lysate
Amino Acid Sequence
1MAEGSRGGPT CSGVGGRQDP VSGSGGCNFP EYELPELNTR AFHVGAFGEL WRGRLRGAGD
61LSLREPPASA LPGSQAADSD REDAAVARDL DCSLEAAAEL RAVCGLDKLK CLEDGEDPEV
121IPENTDLVTL GVRKRFLEHR EETITIDRAC RQETFVYEME SHAIGKKPEN SADMIEEGEL
181ILSVNILYPV IFHKHKEHKP YQTMLVLGSQ KLTQLRDSIR CVSDLQIGGE FSNTPDQAPE
241HISKDLYKSA FFYFEGTFYN DKRYPECRDL SRTIIEWSES HDRGYGKFQT ARMEDFTFND
301LCIKLGFPYL YCHQGDCEHV IVITDIRLVH HDDCLDRTLY PLLIKKHWLW TRKCFVCKMY
361TARWVTNNDS FAPEDPCFFC DVCFRMLHYD SEGNKLGEFL AYPYVDPGTF N
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

411 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.