Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SNAP91 / AP180 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113635-100
Catalog Number: LS-G113635
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human AP180(SNAP91) (NM_014841), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Assembly protein | 180kDa | AP180 | CALM | Phosphoprotein F1-20 | KIAA0656 | SNAP91
- Origin Species
- Human
- NCBI GenBank
- AB014556
- SwissProt
- O60641
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 92.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGQTLTDRI AAAQYSVTGS AVARAVCKAT THEVMGPKKK HLDYLIQATN ETNVNIPQMA61DTLFERATNS SWVVVFKALV TTHHLMVHGN ERFIQYLASR NTLFNLSNFL DKSGSHGYDM121STFIRRYSRY LNEKAFSYRQ MAFDFARVKK GADGVMRTMA PEKLLKSMPI LQGQIDALLE181FDVHPNELTN GVINAAFMLL FKDLIKLFAC YNDGVINLLE KFFEMKKGQC KDALEIYKRF241LTRMTRVSEF LKVAEQVGID KGDIPDLTQA PSSLMETLEQ HLNTLEGKKP GNNEGSGAPS301PLSKSSPATT VTSPNSTPAK TIDTSPPVDL FATASAAVPV STSKPSSDLL DLQPDFSSGG361AAAAAAPAPP PPAGGATAWG DLLGEDSLAA LSSVPSEAQI SDPFAPEPTP PTTTAEIATA421SASASTTTTV TAVTAEVDLF GDAFAASPGE APAASEGAAA PATPTPVAAA LDACSGNDPF481APSEGSAEAA PELDLFAMKP PETSVPVVTP TASTAPPVPA TAPSPAPAVA AAAAATTAAT541AAATTTTTTS AATATTAPPA LDIFGDLFES TPEVAAAPKP DAAPSIDLFS TDAFSSPPQG601ASPVPESSLT ADLLSVDAFA APSPATTASP AKVDSSGVID LFGDAFGSSA SEPQPASQAA661SSSSASADLL AGFGGSFMAP SPSPVTPAQN NLLQPNFEAA FGTTPSTSSS SSFDPSVFDG721LGDLLMPTMA PAGQPAPVSM VPPSPAMAAS KALGSDLDSS LASLVGNLGI SGTTTKKGDL781QWNAGEKKLT GGANWQPKVA PATWSAGVPP SAPLQGAVPP TSSVPPVAGA PSVGQPGAGF841GMPPAGTGMP MMPQQPVMFA QPMMRPPFGA AAVPGTQLSP SPTPASQSPK KPPAKDPLAD901LNIKDFLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
907 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.