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SMYD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMYD4 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G108088-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMYD4 (NM_052928), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1936 | SMYD4 | ZMYND21
Origin Species
Human
NCBI GenBank
AI141071
SwissProt
Q8IYR2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
89 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDLPVDEWKS YLLQKWASLP TSVQVTISTA ETLRDIFLHS SSLLQPEDEL FLKRLSKGYL
61VGKDSDAPLF YREEGNKKFQ EKDYTGAAVL YSKGVSHSRP NTEDMSLCHA NRSAALFHLG
121QYETCLKDIN IAQTHGYPER LQPKIMLRKA ECLVALGRLQ EASQTISDLE RNFTATPALA
181DVLPQTLQRN LHRLKMKMQE KDSLTESFPA ALAKTLEDAA LREENEQLSN ASSSIGLCVD
241PLKGRCLVAT KDILPGELLV QEDAFVSVLN PGELPPPHHG LDSKWDTRVT NGDLYCHRCL
301KHTLATVPCD GCSYAKYCSQ ECLQQVWELY HRTECPLGGL LLTLGVFCHI ALRLTLLVGF
361EDVRKIITKL CDKISNKDIC LPESNNQVKT LNYGLGESEK NGNIVETPIP GCDINGKYEN
421NYNAVFNLLP HTENHSPEHK FLCALCVSAL CRQLEAASLQ AIPTERIVNS SQLKAAVTPE
481LCPDVTIWGV AMLRHMLQLQ CNAQAMTTIQ HTGPKGSIVT DSRQVRLATG IFPVISLLNH
541SCSPNTSVSF ISTVATIRAS QRIRKGQEIL HCYGPHKSRM GVAERQQKLR SQYFFDCACP
601ACQTEAHRMA AGPRWEAFCC NSCGAPMQGD DVLRCGSRSC AESAVSRDHL VSRLQDLQQQ
661VRVAQKLLRD GELERAVQRL SGCQRDAESF LWAEHAVVGE IADGLARACA ALGDWQKSAT
721HLQRSLCVVE VRHGPSSVEM GHELFKLAQI FFNGFAVPEA LSTIQKAEEV LSLHCGPWDD
781EIQELQKMKS CLLDLPPTPV GPAL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

804 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.