LSBio
SMYD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMYD1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SMYD1 antibodies →
$725.00each
Catalog Number: LS-G103766-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMYD1 (NM_198274), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BOP | KMT3D | ZMYND22 | ZMYND18 | CD8 beta opposite | SMYD1
Origin Species
Human
NCBI GenBank
NM_198274
SwissProt
Q8NB12

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MTIGRMENVE VFTAEGKGRG LKATKEFWAA DIIFAERAYS AVVFDSLVNF VCHTCFKRQE
61KLHRCGQCKF AHYCDRTCQK DAWLNHKNEC SAIKRYGKVP NENIRLAARI MWRVEREGTG
121LTEGCLVSVD DLQNHVEHFG EEEQKDLRVD VDTFLQYWPP QSQQFSMQYI SHIFGVINCN
181GFTLSDQRGL QAVGVGIFPN LGLVNHDCWP NCTVIFNNGN HEAVKSMFHT QMRIELRALG
241KISEGEELTV SYIDFLNVSE ERKRQLKKQY YFDCTCEHCQ KKLKDDLFLG VKDNPKPSQE
301VVKEMIQFSK DTLEKIDKAR SEGLYHEVVK LCRECLEKQE PVFADTNIYM LRMLSIVSEV
361LSYLQAFEEA SFYARRMVDG YMKLYHPNNA QLGMAVMRAG LTNWHAGNIE VGHGMICKAY
421AILLVTHGPS HPITKDLEAM RVQTEMELRM FRQNEFMYYK MREAALNNQP MQVMAEPSNE
481PSPALFHKKQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

490 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.