Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SMOX / PAO Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G104963-100
Catalog Number: LS-G104963
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMOX (NM_175839), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C20orf16 | DJ779E11.1 | PAO-1 | PAOH1 | PAO | SMOX | Spermine oxidase | Polyamine oxidase 1
- Origin Species
- Human
- NCBI GenBank
- AK000753
- SwissProt
- Q9NWM0
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 61.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MQSCESSGDS ADDPLSRGLR RRGQPRVVVI GAGLAGLAAA KALLEQGFTD VTVLEASSHI61GGRVQSVKLG HATFELGATW IHGSHGNPIY HLAEANGLLE ETTDGERSVG RISLYSKNGV121ACYLTNHGRR IPKDVVEEFS DLYNEVYNLT QEFFRHDKPV NAESQNSVGV FTREEVRNRI181RNDPDDPEAT KRLKLAMIQQ YLKVESCESS SHSMDEVSLS AFGEWTEIPG AHHIIPSGFM241RVVELLAEGI PAHVIQLGKP VRCIHWDQAS ARPRGPEIEP RGEGDHNHDT GEGGQGGEEP301RGGRWDEDEQ WSVVVECEDC ELIPADHVIV TVSLGVLKRQ YTSFFRPGLP TEKVAAIHRL361GIGTTDKIFL EFEEPFWGPE CNSLQFVWED EAESHTLTYP PELWYRKICG FDVLYPPERY421GHVLSGWICG EEALVMEKCD DEAVAEICTE MLRQFTGNPN IPKPRRILRS AWGSNPYFRG481SYSYTQVGSS GADVEKLAKP LPYTESSKTA PMQVLFSGEA THRKYYSTTH GALLSGQREA541ARLIEMYRDL FQQGTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
555 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.