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SMOC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMOC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G101794-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMOC2 (NM_022138), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BA270C4A.1 | BA37D8.1 | DTDP1 | MST117 | MSTP117 | RP11-270C4__A.1 | SMAP-2 | DJ421D16.1 | MSTP140 | SMOC-2 | SMOC2
Origin Species
Human
NCBI GenBank
NM_022138
SwissProt
Q9H3U7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLLPQLCWLP LLAGLLPPVP AQKFSALTFL RVDQDKDKDC SLDCAGSPQK PLCASDGRTF
61LSRCEFQRAK CKDPQLEIAY RGNCKDVSRC VAERKYTQEQ ARKEFQQVFI PECNDDGTYS
121QVQCHSYTGY CWCVTPNGRP ISGTAVAHKT PRCPGSVNEK LPQREGTGKT VSLQIFSVLN
181SDDAAAPALE TQPQGDEEDI ASRYPTLWTE QVKSRQNKTN KNSVSSCDQE HQSALEEAKQ
241PKNDNVVIPE CAHGGLYKPV QCHPSTGYCW CVLVDTGRPI PGTSTRYEQP KCDNTARAHP
301AKARDLYKGR QLQGCPGAKK HEFLTSVLDA LSTDMVHAAS DPSSSSGRLS EPDPSHTLEE
361RVVHWYFKLL DKNSSGDIGK KEIKPFKRFL RKKSKPKKCV KKFVEYCDVN NDKSISVQEL
421MGCLGVAKED GKADTKKRHT PRGHAESTSN RQPRKQG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

457 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.