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SMOC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMOC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SMOC1 antibodies →
$504.00each
Catalog Number: LS-G99296-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMOC1 (NM_001034852), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
OAS | SMOC-1 | SMOC1
NCBI GenBank
NM_022137
SwissProt
Q9H4F8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
48.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLPARCARLL TPHLLLVLVQ LSPARGHRTT GPRFLISDRD PQCNLHCSRT QPKPICASDG
61RSYESMCEYQ RAKCRDPTLG VVHRGRCKDA GQSKCRLERA QALEQAKKPQ EAVFVPECGE
121DGSFTQVQCH TYTGYCWCVT PDGKPISGSS VQNKTPVCSG SVTDKPLSQG NSGRKDDGSK
181PTPTMETQPV FDGDEITAPT LWIKHLVIKD SKLNNTNIRN SEKVYSCDQE RQSALEEAQQ
241NPREGIVIPE CAPGGLYKPV QCHQSTGYCW CVLVDTGRPL PGTSTRYVMP SCESDARAKT
301TEADDPFKDR ELPGCPEGKK MEFITSLLDA LTTDMVQAIN SAAPTGGGRF SEPDPSHTLE
361ERVVHWYFSQ LDSNSSNDIN KREMKPFKRY VKKKAKPKKC ARRFTDYCDL NKDKVISLPE
421LKGCLGVSKE VGRLV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

435 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.