Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SMMHC / MYH11 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120250-100
Catalog Number: LS-G120250
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human smooth muscle Myosin heavy chain 11(MYH11) (NM_001040114), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- AAT4 | FAA4 | KIAA0866 | Myosin-11 | MYH11 | Myosin heavy chain 11 | SMHC | SMMHC
- Origin Species
- Human
- NCBI GenBank
- NM_002474
- SwissProt
- P35749
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 227.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAQKGQLSDD EKFLFVDKNF INSPVAQADW AAKRLVWVPS EKQGFEAASI KEEKGDEVVV61ELVENGKKVT VGKDDIQKMN PPKFSKVEDM AELTCLNEAS VLHNLRERYF SGLIYTYSGL121FCVVVNPYKH LPIYSEKIVD MYKGKKRHEM PPHIYAIADT AYRSMLQDRE DQSILCTGES181GAGKTENTKK VIQYLAVVAS SHKGKKDTSI TQGPSFAYGE LEKQLLQANP ILEAFGNAKT241VKNDNSSRFG KFIRINFDVT GYIVGANIET YLLEKSRAIR QARDERTFHI FYYMIAGAKE301KMRSDLLLEG FNNYTFLSNG FVPIPAAQDD EMFQETVEAM AIMGFSEEEQ LSILKVVSSV361LQLGNIVFKK ERNTDQASMP DNTAAQKVCH LMGINVTDFT RSILTPRIKV GRDVVQKAQT421KEQADFAVEA LAKATYERLF RWILTRVNKA LDKTHRQGAS FLGILDIAGF EIFEVNSFEQ481LCINYTNEKL QQLFNHTMFI LEQEEYQREG IEWNFIDFGL DLQPCIELIE RPNNPPGVLA541LLDEECWFPK ATDKSFVEKL CTEQGSHPKF QKPKQLKDKT EFSIIHYAGK VDYNASAWLT601KNMDPLNDNV TSLLNASSDK FVADLWKDVD RIVGLDQMAK MTESSLPSAS KTKKGMFRTV661GQLYKEQLGK LMTTLRNTTP NFVRCIIPNH EKRSGKLDAF LVLEQLRCNG VLEGIRICRQ721GFPNRIVFQE FRQRYEILAA NAIPKGFMDG KQACILMIKA LELDPNLYRI GQSKIFFRTG781VLAHLEEERD LKITDVIMAF QAMCRGYLAR KAFAKRQQQL TAMKVIQRNC AAYLKLRNWQ841WWRLFTKVKP LLQVTRQEEE MQAKEDELQK TKERQQKAEN ELKELEQKHS QLTEEKNLLQ901EQLQAETELY AEAEEMRVRL AAKKQELEEI LHEMEARLEE EEDRGQQLQA ERKKMAQQML961DLEEQLEEEE AARQKLQLEK VTAEAKIKKL EDEILVMDDQ NNKLSKERKL LEERISDLTT1021NLAEEEEKAK NLTKLKNKHE SMISELEVRL KKEEKSRQEL EKLKRKLEGD ASDFHEQIAD1081LQAQIAELKM QLAKKEEELQ AALARLDDEI AQKNNALKKI RELEGHISDL QEDLDSERAA1141RNKAEKQKRD LGEELEALKT ELEDTLDSTA TQQELRAKRE QEVTVLKKAL DEETRSHEAQ1201VQEMRQKHAQ AVEELTEQLE QFKRAKANLD KNKQTLEKEN ADLAGELRVL GQAKQEVEHK1261KKKLEAQVQE LQSKCSDGER ARAELNDKVH KLQNEVESVT GMLNEAEGKA IKLAKDVASL1321SSQLQDTQEL LQEETRQKLN VSTKLRQLEE ERNSLQDQLD EEMEAKQNLE RHISTLNIQL1381SDSKKKLQDF ASTVEALEEG KKRFQKEIEN LTQQYEEKAA AYDKLEKTKN RLQQELDDLV1441VDLDNQRQLV SNLEKKQRKF DQLLAEEKNI SSKYADERDR AEAEAREKET KALSLARALE1501EALEAKEELE RTNKMLKAEM EDLVSSKDDV GKNVHELEKS KRALETQMEE MKTQLEELED1561ELQATEDAKL RLEVNMQALK GQFERDLQAR DEQNEEKRRQ LQRQLHEYET ELEDERKQRA1621LAAAAKKKLE GDLKDLELQA DSAIKGREEA IKQLRKLQAQ MKDFQRELED ARASRDEIFA1681TAKENEKKAK SLEADLMQLQ EDLAAAERAR KQADLEKEEL AEELASSLSG RNALQDEKRR1741LEARIAQLEE ELEEEQGNME AMSDRVRKAT QQAEQLSNEL ATERSTAQKN ESARQQLERQ1801NKELRSKLHE MEGAVKSKFK STIAALEAKI AQLEEQVEQE AREKQAATKS LKQKDKKLKE1861ILLQVEDERK MAEQYKEQAE KGNARVKQLK RQLEEAEEES QRINANRRKL QRELDEATES1921NEAMGREVNA LKSKLRRGNE TSFVPSRRSG GRRVIENADG SEEETDTRDA DFNGTKASETRTRPLEQKLISEEDLAANDILDYKDDDDKV
1979 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.