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SMG9 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMG9 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SMG9 antibodies →
$725.00each
Catalog Number: LS-G111357-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C19orf61(SMG9) (NM_019108), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C19orf61 | F17127_1 | Protein smg-9 homolog | Protein SMG9 | SMG9
Origin Species
Human
NCBI GenBank
NM_019108
SwissProt
Q9H0W8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
57.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAIDEEGGR EIGDEVNILV KEQTQLGVKT LMRLLKEPEK ERDSDSDFSP LQQTEGCQRR
61DKHFRHAENP HHPLKTSSRA APLEKPIVLM KPREEGKGPV AVTGASTPEG TAPPPPAAPA
121PPKGEKEGQR PTQPVYQIQN RGMGTAAPAA MDPVVGQAKL LPPERMKHSI KLVDDQMNWC
181DSAIEYLLDQ TDVLVVGVLG LQGTGKSMVM SLLSANTPEE DQRTYVFRAQ SAEMKERGGN
241QTSGIDFFIT QERIVFLDTQ PILSPSILDH LINNDRKLPP EYNLPHTYVE MQSLQIAAFL
301FTVCHVVIVV QDWFTDLSLY RLWDLGCKCK SNSHSPQTPR FLQTAEMVKP STPSPSHESS
361SSSGSDEGTE YYPHLVFLQN KARREDFCPR KLRQMHLMID QLMAHSHLRY KGTLSMLQCN
421VFPGLPPDFL DSEVNLFLVP FMDSEAESEN PPRAGPGSSP LFSLLPGYRG HPSFQSLVSK
481LRSQVMSMAR PQLSHTILTE KNWFHYAARI WDGVRKSSAL AEYSRLLA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

528 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.