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SMG7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMG7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G130391-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMG7 (NM_001174061), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
EST1-like protein C | EST1C | Ever shorter telomeres 1C | HSMG-7 | SGA56M | SMG-7 | SMG7 | C1orf16 | KIAA0250 | Protein SMG7 | SMG-7 homolog
Origin Species
Human
NCBI GenBank
NM_173156
SwissProt
Q92540

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLVTDLEYAL DKKVEQDLWN HAFKNQITTL QGQAKNRANP NRSEVQANLS LFLEAASGFY
61TQLLQELCTV FNVDLPCRVK SSQLGIISNK QTHTSAIVKP QSSSCSYICQ HCLVHLGDIA
121RYRNQTSQAE SYYRHAAQLV PSNGQPYNQL AILASSKGDH LTTIFYYCRS IAVKFPFPAA
181STNLQKALSK ALESRDEVKT KWGVSDFIKA FIKFHGHVYL SKSLEKLSPL REKLEEQFKR
241LLFQKAFNSQ QLVHVTVINL FQLHHLRDFS NETEQHTYSQ DEQLCWTQLL ALFMSFLGIL
301CKCPLQNESQ EESYNAYPLP AVKVSMDWLR LRPRVFQEAV VDERQYIWPW LISLLNSFHP
361HEEDLSSISA TPLPEEFELQ GFLALRPSFR NLDFSKGHQG ITGDKEGQQR RIRQQRLISI
421GKWIADNQPR LIQCENEVGK LLFITEIPEL ILEDPSEAKE NLILQETSVI ESLAADGSPG
481LKSVLSTSRN LSNNCDTGEK PVVTFKENIK TREVNRDQGR SFPPKEVRRD YSKGITVTKN
541DGKKDNNKRK TETKKCTLEK LQETGKQNVA VQVKSQTELR KTPVSEARKT PVTQTPTQAS
601NSQFIPIHHP GAFPPLPSRP GFPPPTYVIP PPVAFSMGSG YTFPAGVSVP GTFLQPTAHS
661PAGNQVQAGK QSHIPYSQQR PSGPGPMNQG PQQSQPPSQQ PLTSLPAQPT AQSTSQLQVQ
721ALTQQQQSPT KAVPALGKSP PHHSGFQQYQ QADASKQLWN PPQVQGPLGK IMPVKQPYYL
781QTQDPIKLFE PSLQPPVMQQ QPLEKKMKPF PMEPYNHNPS EVKVPEFYWD SSYSMADNRS
841VMAQQANIDR RGKRSPGVFR PEQDPVPRMP FEDPKSSPLL PPDLLKSLAA LEEEEELIFS
901NPPDLYPALL GPLASLPGRS LFKSLLEKPS ELMSHSSSFL SLTGFSLNQE RYPNNSMFNE
961VYGKNLTSSS KAELSPSMAP QETSLYSLFE GTPWSPSLPA SSDHSTPASQ SPHSSNPSSL
1021PSSPPTHNHN SVPFSNFGPI GTPDNRDRRT ADRWKTDKPA MGGFGIDYLS ATSSSESSWH
1081QASTPSGTWT GHGPSMEDSS AVLMESLKSI WSSSMMHPGP SALEQLLMQQ KQKQQRGQGT
1141MNPPH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1145 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.