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SMEK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMEK2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G111149-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMEK2(PPP4R3B) (NM_020463), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1387 | SMEK2 | Smk1 | PP4R3B | PPP4R3B | FLFL2 | PSY2 | SMEK homolog 2
Origin Species
Human
NCBI GenBank
AB037808
SwissProt
Q5MIZ7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSDTRRRVKV YTLNEDRQWD DRGTGHVSST YVEELKGMSL LVRAESDGSL LLESKINPNT
61AYQKQQDTLI VWSEAENYDL ALSFQEKAGC DEIWEKICQV QGKDPSVEVT QDLIDESEEE
121RFEEMPETSH LIDLPTCELN KLEEIADLVT SVLSSPIRRE KLALALENEG YIKKLLQLFQ
181ACENLENTEG LHHLYEIIRG ILFLNKATLF EVMFSDECIM DVVGCLEYDP ALAQPKRHRE
241FLTKTAKFKE VIPITDSELR QKIHQTYRVQ YIQDIILPTP SVFEENFLST LTSFIFFNKV
301EIVSMLQEDE KFLSEVFAQL TDEATDDDKR RELVNFFKEF CAFSQTLQPQ NRDAFFKTLA
361KLGILPALEI VMGMDDLQVR SAATDIFSYL VEFSPSMVRE FVMQEAQQSD DDILLINVVI
421EQMICDTDPE LGGAVQLMGL LRTLIDPENM LATTNKTEKS EFLNFFYNHC MHVLTAPLLT
481NTSEDKCEKD NIVGSNKNNT ICPGALRFMR RIIGLKDEFY NRYITKGNLF EPVINALLDN
541GTRYNLLNSA VIELFEFIRV EDIKSLTAHI VENFYKALES IEYVQTFKGL KTKYEQEKDR
601QNQKLNSVPS ILRSNRFRRD AKALEEDEEM WFNEDEEEEG KAVVAPVEKP KPEDDFPDNY
661EKFMETKKAK ESEDKENLPK RTSPGGFKFT FSHSASAANG TNSKSVVAQI PPATSNGSSS
721KTTNLPTSVT ATKGSLVGLV DYPDDEEEDE EEESSPRKRP RLGS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

764 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.