LSBio
SMCO3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMCO3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G121538-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C12orf69(SMCO3) (NM_001013698), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C12orf69 | RIKEN cDNA C030030A07 gene | LOC440087 | LOC632209
Origin Species
Human
NCBI GenBank
NM_001013698
SwissProt
A2RU48

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
24.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAQSDFLYPE NPKRREEVNR LHQQLLDCLS DSFDVTNKLT EVLNMHLGCR LASIEMKRDG
61TIKENCDLII QAIMKIQKEL QKVDEALKDK LEPTLYRKLQ DIKEKETDKI AIVQKVISVI
121LGEATSAASA VAVKLVGSNV TTGIINKLVT VLAQIGASLL GSIGVAVLGL GIDMIVRAIL
181GAVEKTQLQA AIKSYEKHLV EFKSASEKYN HAITEVINSE TPNEMNSRFI CH
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

232 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.