Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SMCO2 / C12orf70 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G126474-100
Catalog Number: LS-G126474
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C12orf70(SMCO2) (NM_001145010), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001145010
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 39.3 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MALTPTNLNN KMSLQMKMDC QEQQLTKKNN GFFQKLNVTE GAMQDLLKEI IKVDHILDRS61DDEDDISSEN PQTDFLHKGM LELEAEHDQD LSKQDKQETD VDEDPQASTS LQFSKKNLLE121LCLKGMFLKL NYWNTKIGLQ VKELGADYID GTEKIDNIIK KINVTENTVK SLLKDMLTLK181GQIEKLEDRG LDLDQGTSTE VNTCNEVYEL KKKVIERLED LCKNVELLSA KLRMYQMEAE241DTDSHSSEEI DTEEMEALLP QAPASFLVQK SPPRNTAWKR ALRIFIMFDV LTVTGLLCYI301LFFGATFLFE RVLLRMLGCR TTWDLREMRE PFLNLEVEAL LPSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
343 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.