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SMC6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMC6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SMC6 antibodies →
$725.00each
Catalog Number: LS-G125847-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMC6L1(SMC6) (NM_001142286), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HSMC6 | SMC6 | SMC protein 6 | SMC-6 | SMC6L1
Origin Species
Human
NCBI GenBank
NM_024624
SwissProt
Q96SB8

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
126.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAKRKEENFS SPKNAKRPRQ EELEDFDKDG DEDECKGTTL TAAEVGIIES IHLKNFMCHS
61MLGPFKFGSN VNFVVGNNGS GKSAVLTALI VGLGGRAVAT NRGSSLKGFV KDGQNSADIS
121ITLRNRGDDA FKASVYGNSI LIQQHISIDG SRSYKLKSAT GSVVSTRKEE LIAILDHFNI
181QVDNPVSVLT QEMSKQFLQS KNEGDKYKFF MKATQLEQMK EDYSYIMETK ERTKEQIHQG
241EERLTELKRQ CVEKEERFQS IAGLSTMKTN LESLKHEMAW AVVNEIEKQL NAIRDNIKIG
301EDRAARLDRK MEEQQVRLNE AEQKYKDIQD KLEKISEETN ARAPECMALK ADVVAKKRAY
361NEAEVLYNRS LNEYKALKKD DEQLCKRIEE LKKSTDQSLE PERLERQKKI SWLKERVKAF
421QNQENSVNQE IEQFQQAIEK DKEEHGKIKR EELDVKHALS YNQRQLKELK DSKTDRLKRF
481GPNVPALLEA IDDAYRQGHF TYKPVGPLGA CIHLRDPELA LAIESCLKGL LQAYCCHNHA
541DERVLQALMK RFYLPGTSRP PIIVSEFRNE IYDVRHRAAY HPDFPTVLTA LEIDNAVVAN
601SLIDMRGIET VLLIKNNSVA RAVMQSQKPP KNCREAFTAD GDQVFAGRYY SSENTRPKFL
661SRDVDSEISD LENEVENKTA QILNLQQHLS ALEKDIKHNE ELLKRCQLHY KELKMKIRKN
721ISEIRELENI EEHQSVDIAT LEDEAQENKS KMKMVEEHME QQKENMEHLK SLKIEAENKY
781DAIKFKINQL SELADPLKDE LNLADSEVDN QKRGKRHYEE KQKEHLDTLN KKKRELDMKE
841KELEEKMSQA RQICPERIEV EKSASILDKE INRLRQKIQA EHASHGDREE IMRQYQEARE
901TYLDLDSKVR TLKKFIKLLG EIMEHRFKTY QQFRRCLTLR CKLYFDNLLS QRAYCGKMNF
961DHKNETLSIS VQPGEGNKAA FNDMRALSGG ERSFSTVCFI LSLWSIAESP FRCLDEFDVY
1021MDMVNRRIAM DLILKMADSQ RFRQFILLTP QSMSSLPSSK LIRILRMSDP ERGQTTLPFR
1081PVTQEEDDDQ R
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1091 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.