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SMARCC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SMARCC2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G107121-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SMRC2(SMARCC2) (NM_139067), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BRG1-associated factor 170 | CRACC2 | SMARCC2 | SWI3-like protein | BAF170 | Rsc8
Origin Species
Human
NCBI GenBank
NM_003075
SwissProt
Q8TAQ2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
124.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAVRKKDGGP NVKYYEAADT VTQFDNVRLW LGKNYKKYIQ AEPPTNKSLS SLVVQLLQFQ
61EEVFGKHVSN APLTKLPIKC FLDFKAGGSL CHILAAAYKF KSDQGWRRYD FQNPSRMDRN
121VEMFMTIEKS LVQNNCLSRP NIFLCPEIEP KLLGKLKDII KRHQGTVTED KNNASHVVYP
181VPGNLEEEEW VRPVMKRDKQ VLLHWGYYPD SYDTWIPASE IEASVEDAPT PEKPRKVHAK
241WILDTDTFNE WMNEEDYEVN DDKNPVSRRK KISAKTLTDE VNSPDSDRRD KKGGNYKKRK
301RSPSPSPTPE AKKKNAKKGP STPYTKSKRG HREEEQEDLT KDMDEPSPVP NVEEVTLPKT
361VNTKKDSESA PVKGGTMTDL DEQEDESMET TGKDEDENST GNKGEQTKNP DLHEDNVTEQ
421THHIIIPSYA AWFDYNSVHA IERRALPEFF NGKNKSKTPE IYLAYRNFMI DTYRLNPQEY
481LTSTACRRNL AGDVCAIMRV HAFLEQWGLI NYQVDAESRP TPMGPPPTSH FHVLADTPSG
541LVPLQPKTPQ GRQVDADTKA GRKGKELDDL VPETAKGKPE LQTSASQQML NFPDKGKEKP
601TDMQNFGLRT DMYTKKNVPS KSKAAASATR EWTEQETLLL LEALEMYKDD WNKVSEHVGS
661RTQDECILHF LRLPIEDPYL EDSEASLGPL AYQPIPFSQS GNPVMSTVAF LASVVDPRVA
721SAAAKSALEE FSKMKEEVPT ALVEAHVRKV EEAAKVTGKA DPAFGLESSG IAGTTSDEPE
781RIEESGNDEA RVEGQATDEK KEPKEPREGG GAIEEEAKEK TSEAPKKDEE KGKEGDSEKE
841SEKSDGDPIV DPEKEKEPKE GQEEVLKEVV ESEGERKTKV ERDIGEGNLS TAAAAALAAA
901AVKAKHLAAV EERKIKSLVA LLVETQMKKL EIKLRHFEEL ETIMDREREA LEYQRQQLLA
961DRQAFHMEQL KYAEMRARQQ HFQQMHQQQQ QPPPALPPGS QPIPPTGAAG PPAVHGLAVA
1021PASVVPAPAG SGAPPGSLGP SEQIGQAGST AGPQQQQPAG APQPGAVPPG VPPPGPHGPS
1081PFPNQQTPPS MMPGAVPGSG HPGVADPGTP LPPDPTAPSP GTVTPVPPPQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1130 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.