Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLX4IP / C20orf94 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G121450-100
Catalog Number: LS-G121450
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLX4IP (NM_001009608), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BA204H22.1 | C20orf94 | DJ1099D15.3 | SLX4 interacting protein | SLX4IP | BA254M13.1
- Origin Species
- Human
- NCBI GenBank
- NM_001009608
- SwissProt
- Q5VYV7
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 45.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MASKKFAVKC GNFAVLVDLH ILPQGSNKDT SWFSEQKKEE VCLLLKETID SRVQEYLEVR61KQHRPSNAEF TRSNPLSLKG YGFQITAYFL KRGIRLRCIR STQNAELCVF PDRFVVCVSQ121LAFSRDLLAS QNEDLTERVL HGVSDYFAEC AESSLPPSAK LRRNALKEIV KRTETKSSVT181SKSQTRRDTV ETSSDSVIAE IARRRNDGQA SSSPPSESMG QAKDSIKAAE SHWGLPVQKL241EKVNQTQPED TSGQQKPHPG ERLKTGLLSR SPVCSCESAS PCPKQSPRVA KTQQKRRNCS301SAEDFDHHGR VSLGSDRLVP REIIVEKSKA VRVLPASELS DPGLLLKQDL AKTTSKEELH361VLESLSSRHL MKNNPGQAQQ TGLATNTERL STIQNSPTKK RKKYERGHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
408 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.