Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLMAP / SLAP Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G114828-100
Catalog Number: LS-G114828
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLMAP (NM_007159), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- KIAA1601 | Sarcolemma associated protein | SLAP | SLMAP | Sarcolemmal-associated protein
- Origin Species
- Human
- NCBI GenBank
- NM_007159
- SwissProt
- Q14BN4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 93 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPSALAIFTC RPNSHPFQER HVYLDEPIKI GRSVARCRPA QNNATFDCKV LSRNHALVWF61DHKTGKFYLQ DTKSSNGTFI NSQRLSRGSE ESPPCEILSG DIIQFGVDVT ENTRKVTHGC121IVSTIKLFLP DGMEARLRSD VIHAPLPSPV DKVAANTPSM YSQELFQLSQ YLQEALHREQ181MLEQKLATLQ RLLAITQEAS DTSWQALIDE DRLLSRLEVM GNQLQACSKN QTEDSLRKEL241IALQEDKHNY ETTAKESLRR VLQEKIEVVR KLSEVERSLS NTEDECTHLK EMNERTQEEL301RELANKYNGA VNEIKDLSDK LKVAEGKQEE IQQKGQAEKK ELQHKIDEME EKEQELQAKI361EALQADNDFT NERLTALQEH LLSKSGGDCT FIHQFIECQK KLIVEGHLTK AVEETKLSKE421NQTRAKESDF SDTLSPSKEK SSDDTTDAQM DEQDLNEPLA KVSLLKDDLQ GAQSEIEAKQ481EIQHLRKELI EAQELARTSK QKCFELQALL EEERKAYRNQ VEESTKQIQV LQAQLQRLHI541DTENLREEKD SEITSTRDEL LSARDEILLL HQAAAKVASE RDTDIASLQE ELKKVRAELE601RWRKAASEYE KEITSLQNSF QLRCQQCEDQ QREEATRLQG ELEKLRKEWN ALETECHSLK661RENVLLSSEL QRQEKELHNS QKQSLELTSD LSILQMSRKE LENQVGSLKE QHLRDSADLK721TLLSKAENQA KDVQKEYEKT QTVLSELKLK FEMTEQEKQS ITDELKQCKN NLKLLREKGN781NKPWPWMPML AALVAVTAIV LYVPGLARAS PTRTRPLEQKLISEEDLAANDILDYKDDDDKV
811 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.