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SLITRK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SLITRK3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G113600-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLITRK3 (NM_014926), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA0848 | Slit and trk like gene 3 | SLITRK3 | SLIT and NTRK-like protein 3
Origin Species
Human
NCBI GenBank
NM_014926
SwissProt
O94933

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
108.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKPSIAEMLH RGRMLWIILL STIALGWTTP IPLIEDSEEI DEPCFDPCYC EVKESLFHIH
61CDSKGFTNIS QITEFWSRPF KLYLQRNSMR KLYTNSFLHL NNAVSINLGN NALQDIQTGA
121FNGLKILKRL YLHENKLDVF RNDTFLGLES LEYLQADYNV IKRIESGAFR NLSKLRVLIL
181NDNLIPMLPT NLFKAVSLTH LDLRGNRLKV LFYRGMLDHI GRSLMELQLE ENPWNCTCEI
241VQLKSWLERI PYTALVGDIT CETPFHFHGK DLREIRKTEL CPLLSDSEVE ASLGIPHSSS
301SKENAWPTKP SSMLSSVHFT ASSVEYKSSN KQPKPTKQPR TPRPPSTSQA LYPGPNQPPI
361APYQTRPPIP IICPTGCTCN LHINDLGLTV NCKERGFNNI SELLPRPLNA KKLYLSSNLI
421QKIYRSDFWN FSSLDLLHLG NNRISYVQDG AFINLPNLKS LFLNGNDIEK LTPGMFRGLQ
481SLHYLYFEFN VIREIQPAAF SLMPNLKLLF LNNNLLRTLP TDAFAGTSLA RLNLRKNYFL
541YLPVAGVLEH LNAIVQIDLN ENPWDCTCDL VPFKQWIETI SSVSVVGDVL CRSPENLTHR
601DVRTIELEVL CPEMLHVAPA GESPAQPGDS HLIGAPTSAS PYEFSPPGGP VPLSVLILSL
661LVLFFSAVFV AAGLFAYVLR RRRKKLPFRS KRQEGVDLTG IQMQCHRLFE DGGGGGGGSG
721GGGRPTLSSP EKAPPVGHVY EYIPHPVTQM CNNPIYKPRE EEEVAVSSAQ EAGSAERGGP
781GTQPPGMGEA LLGSEQFAET PKENHSNYRT LLEKEKEWAL AVSSSQLNTI VTVNHHHPHH
841PAVGGVSGVV GGTGGDLAGF RHHEKNGGVV LFPPGGGCGS GSMLLDRERP QPAPCTVGFV
901DCLYGTVPKL KELHVHPPGM QYPDLQQDAR LKETLLFSAG KGFTDHQTQK SDYLELRAKL
961QTKPDYLEVL EKTTYRF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

977 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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