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SLFN14 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SLFN14 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G124895-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLFN14 (NM_001129820), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
SLFN14 | Schlafen family member 14
Origin Species
Human
SwissProt
P0C7P3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
103.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESLKTDTEM PYPEVIVDVG RVIFGEENRK KMTNSCLKRS ENSRIIRAIC ALLNSGGGVI
61KAEIDDKTYS YQCHGLGQDL ETSFQKLLPS GSQKYLDYMQ QGHNLLIFVK SWSPDVFSLP
121LRICSLRSNL YRRDVTSAIN LSASSALELL REKGFRAQRG RPRVKKLHPQ QVLNRCIQEE
181EDMRILASEF FKKDKLMYKE KLNFTESTHV EFKRFTTKKV IPRIKEMLPH YVSAFANTQG
241GYVLIGVDDK SKEVVGCKWE KVNPDLLKKE IENCIEKLPT FHFCCEKPKV NFTTKILNVY
301QKDVLDGYVC VIQVEPFCCV VFAEAPDSWI MKDNSVTRLT AEQWVVMMLD TQSAPPSLVT
361DYNSCLISSA SSARKSPGYP IKVHKFKEAL QRHLFPVTQE EVQFKPESLC KKLFSDHKEL
421EGLMKTLIHP CSQGIVIFSR SWAGDVGFRK EQNVLCDALL IAVNSPVVLY TILIDPNWPG
481GLEYARNTAH QLKQKLQTVG GYTGKVCIIP RLIHLSSTQS RPGEIPLRYP RSYRLADEEE
541MEDLLQALVV VSLSSRSLLS DQMGCEFFNL LIMEQSQLLS ESLQKTRELF IYCFPGVRKT
601ALAIKIMEKI KDLFHCKPKE ILYVCESDSL KDFVTQQTTC QAVTRKTFMQ GEFLKIKHIV
661MDETENFCSK YGNWYMKAKN ITHPKAKGTG SENLHHGILW LFLDPFQIHH ADVNGLPPPS
721AQFPRKTITS GIHCALEIAK VMKEEMKRIK ENPPSNMSPD TLALFSETAY EEATCAQALP
781GVCETKTNLT TEQIANYVAR KCHSLFQCGY LPKDIAILCR RGEDRGRYRL ALLKAMELIE
841THRPSEVVFS PATGVWGSHI VLDSIQQFSG LERTVVFGLS PECDQSEEFH KLCFASRAIK
901HLYLLYEKRA AY
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

912 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.