Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLFN13 / FLJ31952 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106932-100
Catalog Number: LS-G106932
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLFN13 (NM_144682), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- SLFN10 | SLFN13 | FLJ31952 | Schlafen family member 13
- Origin Species
- Human
- NCBI GenBank
- AA969331
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 101.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEANHCSLGV YPSYPDLVID VGEVTLGEEN RKKLQKTQRD QERARVIRAA CALLNSGGGV61IQMEMANRDE RPTEMGLDLE ESLRKLIQYP YLQAFFETKQ HGRCFYIFVK SWSGDPFLKD121GSFNSRICSL SSSLYCRSGT SVLHMNSRQA FDFLKTKERQ SKYNLINEGS PPSKIMKAVY181QNISESNPAY EVFQTDTIEY GEILSFPESP SIEFKQFSTK HIQQYVENII PEYISAFANT241EGGYLFIGVD DKSRKVLGCA KEQVDPDSLK NVIARAISKL PIVHFCSSKP RVEYSTKIVE301VFCGKELYGY LCVIKVKAFC CVVFSEAPKS WMVREKYIRP LTTEEWVEKM MDADPEFPPD361FAEAFESQLS LSDSPSLCRP VYSKKGLEHK ADLQQHLFPV PPGHLECTPE SLWKELSLQH421EGLKELIHKQ MRPFSQGIVI LSRSWAVDLN LQEKPGVICD ALLIAQNSTP ILYTILREQD481AEGQDYCTRT AFTLKQKLVN MGGYTGKVCV RAKVLCLSPE SSTEALEAAV SPMDYPASYS541LAGTQHMEAL LQSLVIVLLG FRSLLSDQLG CEVLNLLTAQ QYEIFSRSLR KNRELFVHGL601PGSGKTIMAM KIMEKIRNVF HCEAHRILYV CENQPLRNFI SDRNICRAET RKTFLRENFE661HIQHIVIDEA QNFRTEDGDW YRKAKTITQR EKDCPGVLWI FLDYFQTSHL GHSGLPPLSA721QYPREELTRV VRNADEIAEY IQQEMQLIIE NPPINIPHGY LAILSEAKWV PGVPGNTKII781KNFTLEQIVT YVADTCRCFF ERGYSPKDVA VLVSTVTEVE QYQSKLLKAM RKKMVVQLSD841ACDMLGVHIV LDSVRRFSGL ERSIVFGIHP RTADPAILPN ILICLASRAK QHLYIFLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
897 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.