LSBio
SLFN12 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

SLFN12 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all SLFN12 antibodies →
$504.00each
Catalog Number: LS-G112027-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLFN12 (NM_018042), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Schlafen family member 12 | SLFN3 | FLJ10260 | SLFN12
Origin Species
Human
NCBI GenBank
NM_018042
SwissProt
Q8IYM2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
66.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MNISVDLETN YAELVLDVGR VTLGENSRKK MKDCKLRKKQ NERVSRAMCA LLNSGGGVIK
61AEIENEDYSY TKDGIGLDLE NSFSNILLFV PEYLDFMQNG NYFLIFVKSW SLNTSGLRIT
121TLSSNLYKRD ITSAKVMNAT AALEFLKDMK KTRGRLYLRP ELLAKRPRVD IQEENNMKAL
181AGVFFDRTEL DRKEKLTFTE STHVEIKNFS TEKLLQRIKE ILPQYVSAFA NTDGGYLFIG
241LNEDKEIIGF KAEMSDLDDL EREIEKSIRK MPVHHFCMEK KKINYSCKFL GVYDKGSLCG
301YVCALRVERF CCAVFAKEPD SWHVKDNRVM QLTRKEWIQF MVEAEPKFSS SYEEVISQIN
361TSLPAPHSWP LLEWQRQRHH CPGLSGRITY TPENLCRKLF LQHEGLKQLI CEEMDSVRKG
421SLIFSRSWSV DLGLQENHKV LCDALLISQD SPPVLYTFHM VQDEEFKGYS TQTALTLKQK
481LAKIGGYTKK VCVMTKIFYL SPEGMTSCQY DLRSQVIYPE SYYFTRRKYL LKALFKALKR
541LKSLRDQFSF AENLYQIIGI DCFQKNDKKM FKSCRRLT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

578 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.