Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLCO1C1 / OATPF Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112463-100
Catalog Number: LS-G112463
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SLCO1C1 (NM_017435), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Oatp1 | Organic anion transporter F | SLCO1C1 | Thyroxine transporter | OAT-RP-5 | OATP14 | SLC21A14 | OATP-14 | OATP-F | OATP1C1 | OATPF | OATPRP5
- Origin Species
- Human
- NCBI GenBank
- NM_017435
- SwissProt
- Q9NYB5
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDTSSKENIQ LFCKTSVQPV GRPSFKTEYP SSEEKQPCCG ELKVFLCALS FVYFAKALAE61GYLKSTITQI ERRFDIPSSL VGVIDGSFEI GNLLVITFVS YFGAKLHRPK IIGAGCVIMG121VGTLLIAMPQ FFMEQYKYER YSPSSNSTLS ISPCLLESSS QLPVSVMEKS KSKISNECEV181DTSSSMWIYV FLGNLLRGIG ETPIQPLGIA YLDDFASEDN AAFYIGCVQT VAIIGPIFGF241LLGSLCAKLY VDIGFVNLDH ITITPKDPQW VGAWWLGYLI AGIISLLAAV PFWYLPKSLP301RSQSREDSNS SSEKSKFIID DHTDYQTPQG ENAKIMEMAR DFLPSLKNLF GNPVYFLYLC361TSTVQFNSLF GMVTYKPKYI EQQYGQSSSR ANFVIGLINI PAVALGIFSG GIVMKKFRIS421VCGAAKLYLG SSVFGYLLFL SLFALGCENS DVAGLTVSYQ GTKPVSYHER ALFSDCNSRC481KCSETKWEPM CGENGITYVS ACLAGCQTSN RSGKNIIFYN CTCVGIAASK SGNSSGIVGR541CQKDNGCPQM FLYFLVISVI TSYTLSLGGI PGYILLLRCI KPQLKSFALG IYTLAIRVLA601GIPAPVYFGV LIDTSCLKWG FKRCGSRGSC RLYDSNVFRH IYLGLTVILG TVSILLSIAV661LFILKKNYVS KHRSFITKRE RTMVSTRFQK ENYTTSDHLL QPNYWPGKET QLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
712 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.