Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
SLCO1B1 / OATP2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G100822-100
Catalog Number: LS-G100822
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human OATP2(SLCO1B1) (NM_006446), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- OATP-2 | Oatp2 | SLCO1B1 | OATP-C | OATPC | ORGANIC ANION TRANSPORTER C | RLST-1 | OATP1B1 | SLC21A6
- Origin Species
- Human
- NCBI GenBank
- NM_006446
- SwissProt
- Q9Y6L6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 76.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDQNQHLNKT AEAQPSENKK TRYCNGLKMF LAALSLSFIA KTLGAIIMKS SIIHIERRFE61ISSSLVGFID GSFEIGNLLV IVFVSYFGSK LHRPKLIGIG CFIMGIGGVL TALPHFFMGY121YRYSKETNID SSENSTSTLS TCLINQILSL NRASPEIVGK GCLKESGSYM WIYVFMGNML181RGIGETPIVP LGLSYIDDFA KEGHSSLYLG ILNAIAMIGP IIGFTLGSLF SKMYVDIGYV241DLSTIRITPT DSRWVGAWWL NFLVSGLFSI ISSIPFFFLP QTPNKPQKER KASLSLHVLE301TNDEKDQTAN LTNQGKNITK NVTGFFQSFK SILTNPLYVM FVLLTLLQVS SYIGAFTYVF361KYVEQQYGQP SSKANILLGV ITIPIFASGM FLGGYIIKKF KLNTVGIAKF SCFTAVMSLS421FYLLYFFILC ENKSVAGLTM TYDGNNPVTS HRDVPLSYCN SDCNCDESQW EPVCGNNGIT481YISPCLAGCK SSSGNKKPIV FYNCSCLEVT GLQNRNYSAH LGECPRDDAC TRKFYFFVAI541QVLNLFFSAL GGTSHVMLIV KIVQPELKSL ALGFHSMVIR ALGGILAPIY FGALIDTTCI601KWSTNNCGTR GSCRTYNSTS FSRVYLGLSS MLRVSSLVLY IILIYAMKKK YQEKDINASE661NGSVMDEANL ESLNKNKHFV PSAGADSETH CTRTRPLEQKLISEEDLAANDILDYKDDDDKV
691 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.